Study of proteome enzymes involved in mycoparasitism process of Trichoderma harizanum mutant isolate

Document Type : Research Paper

Authors

1 Plant Protection Department, Gorgan University of Agricultural Sciences and Natural Resources, Golestan, Iran

2 Plant Protection Department, Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), Atomic Energy Organization of Iran (AEOI), Alborz, Iran

Abstract

This study was conducted to investigate the expression pattern of chitinase in mutant strains of the fungus Trichoderma harizanum, to the wildisolate.For this purpose, the strains of T. harizanum were subjected to mutation with optimum doses of 250 Gy of gamma radiation. Both the anti-mutant and wild strains were studiedwith the dual culture on the plant pathogen Sclerotinia sclerotiorum..All statistical analyzes were performed with SAS software and test least significant difference (LSD). The profile protein of produced enzymes by all strains using SDS-PAGE electrophoresis and proteome pattern of wildisolate with Th M8 mutant strains by colloidal chitin substrate was investigated using two-dimensional electrophoresis techniques and software ImageMaster 2D Platinum.The results showed that the majority of mutant strains compared to wild strains in the production of extracellular enzyme chitinase, were significantly different in the 1% and 5%. The results of the test antagonist showed that the potential control mutant strains compared to wild strains of the fungus S. sclerotiorum cope with significantly increased. The highest and lowest inhibition is related to strain ThM8 to 72% and ThM3 to 43%, respectively.On the other hand,the proteome pattern based on, two-dimensional electrophoresis technique showed that the protein spots of mutant strains compared to wild type isolates different both quantitatively and qualitatively.This suggests that mutations can lead to a change in the pattern of expression of the protein is secreted. The results of this study showed that gamma irradiation techniques can be effective in the production of enzymes in maycoparasitism and improvement biological control potential of plant diseases to be effective in management.

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Main Subjects

References

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BioControl in Plant Protection
Volume 6, Issue 2 - Serial Number 12
February 2019
Pages 15-30

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