Preparation of Talaromyces flavus liquid and microcapsule formulations and their application in commercial tomato greenhouses to increase yield

Document Type : Research Paper

Authors

1 Associate Professor, Department of Plant Diseases Research, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran.

2 Assistant Professor,Plant Protection Research Department, Yazd Agricultural and Natural Resources Research and Education Center, AREEO, Yazd, Iran

3 Research instructor, Plant Protection Research Department, Isfahan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Isfahan, Iran.

4 Assistant Professor, Department of Plant Diseases Research, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran.

10.22092/bcpp.2023.364217.355

Abstract

According to the recent advances in the application of biotechnology in various sciences, in recent years, research has been conducted in the field of preparation of liquid bioformulations and microcapsules of suspension and powder from the important fungal inhibitor Talaromyces flavus. In this study, optimization of the mentioned formulations and their use in commercial greenhouses have been done. For this purpose, based on the results of previous greenhouse research, regarding the preparation of a type of liquid bioformulation (liquid formulation containing Potato Dextrose Broth culture medium, temperature of 25 degrees Celsius and dicycloserine or sodium nitrate stabilizer), a type of microcapsule in the form of suspension. A type of microcapsule in powder form was prepared for use in tomato greenhouses. To optimize the liquid formulation, the most suitable emulsifiers and binders in terms of suspensionability were identified as Tween 20 and carboxymethyl cellulose. For the optimization of suspension and powder microcapsules, the most suitable combination of polymer + surfactant in terms of solubility was determined, respectively, the combination of alginate + myristyl alcohol and guargam + calcium lignosulfate. Investigations related to commercial greenhouses were carried out during one year in two regions of Yazd and Isfahan with a history of Fusarium wilt disease. Each study was carried out in the form of a statistical design of complete randomized blocks with eight treatments in four replications. The treatments were: 1 to 3- each of the liquid formulations, suspension microcapsules and powder microcapsules with the method of adding to the soil, 4 to 6- each of the mentioned formulations with the root immersion method, 7- Thalaromin fungicide By the method of adding to the soil, 8- control (without application of formulation and fungicide). The treatments were evaluated by determining the percentage of occurrence and the percentage of severity of fusarium wilt disease. Statistical data analysis and mean comparison was done using Duncan's multiple range test by MS TAT C software program. The results showed that the microcapsule powder with the seedling impregnation method and the liquid formulation with the soil addition method were the most effective treatments by reducing the disease severity percentage by 91.6 and 68.4%, respectively, compared to the control. According to the results Obtained, the method of producing and using each of Talaromyces flavus microcapsule and liquid formulations is considered as technical knowledge and it is possible to proceed with their commercialization steps.

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Main Subjects

References

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BioControl in Plant Protection

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Available Online from 03 February 2024

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