Preparation of concentrated suspension of microencapsulated formulation of Bacillus thuringiensis

Document Type : Research Paper

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Abstract

 In this study, the preparation of microencapsulated Spore Crystal Aggregate (SCA) of  Bacillus thuringiensis

formulation mixed with sodium alginate (2, 3, and 5 %) was investigated. Microencapsulated formulations were prepared using the emulsion gelling procedure. The protective effect of microcapsules after exposure to Ultra Violet radiation (UV) was evaluated by measuring the spore viability and bioassay tests. The use of sodium alginate (5 % w/w) resulted in the highest viabilities as 90 and 86% after exposure to UVB (400nm) and UVC (254nm) irradiation, respectively, while, viability of non- microencapsulated spores under these conditions were 40 and 50%, respectively. The mortality of irradiated and non-irradiated free spore formulations on second instar larvae of Ephestia kuehniella were 15 and 93%, respectively. However, the mortality caused by irradiated and non-irradiated microencapsulated formulations were 70 and 80% on the tenth day of the experiment, respectively. The sizerange of the microcapsules was 7— 20 µm while the microencapsulation efficiency was 86%.

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References

Arthurs, S.P., Lacey, L.A. & Behle, R.W. 2006. Evaluation of spray-dried lignin-based formulations and adjuvants as solar protectants for the granulovirus of the codling moth Cydia  pomonella (L). Journal of Invertebrate Pathology, 93(2): 88–95.
Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Analytical Biochemistry, 72: 248–254.
Burges, H.D. 1998. Formulation of Microbial Biopesticides, Beneficial microorganisms, nematodes and seed treatments. Kluwer Academic, Dordrecht, 412 pp. ISBN: 0-412-62520-2.
Danielle, M.T., Kyle, S., Wayneb, B.H. & Blake, B. 2011. Delivery system using sodium alginate virus loaded pellets to red imported fire ants (Solenopsis invicta, Hymenoptera: formicidae). Florida Entomologist, 94(2):237.
Dubey, R., Shami, T. C. & Bhasker Rao, K.U. 2009. Microencapsulation technology and applications. Defence Science Journal, 59(1): 82-95.
Dunkle, R.L. & Shasha, B.S. 1988. Starch-encapsulated Bacillus thuringiensis: A potential new method for increasing environmental stability of entomopathogens. Environmental Entomology, 17: 120–126.
Griego, V.M. & Spence, K.D. 1978. Inactivation of Bacillus thuringiensis spores by ultraviolet and visible light. Applied and Environmental Microbiology, 35: 906–910.
Karina, G.G., He´ctor, M.V., Fernando, E., Jorge, R. & Josefina, B.C. 2011. Small microcapsules of crystal proteins and spores of Bacillus thuringiensis by an emulsification/internal gelation method. Bioprocess Biosystem Engineering.
Martin, S., Salamouny, S.E.I. & Shepard, B.M. 2009. Plant extracts as ultraviolet radiation protectants for the beet armyworm (Lepidoptera: Noctuidae) nucleopolyhedrovirus: screening of extracts.  Journal of Agricultural and Urban Entomology, 26(2): 47–61.
Martins, S., Sarmento, S.E. & Ferreira, D. 2007. Insulin–loaded alginate microsphere for oral delivery – effect of polysaccharide rein forment on physicochemical properties and release profile. Carbohydrate Polymers, 69: 725–73.
Patricia, T.G., Michael, R.M., Robert, W.B, Baruch, S., & Randall, L.P. 2002. Storage stability of Anagrapha falcifera nucleopolyhedrovirus in spray-dried formulations. Journal of Invertebrate Pathology, 79: 7-16.
Robert, R., Farrar, J.R., Martin, S. & Iqbal, J. 2003. Photostabilized titanium dioxide and a fluorescent brightener as adjuvants for a nucleopolyhedrovirus. BioControl, 48: 543–560.
Rodrigues, A.P., Hirsch, D., Figueiredo, H.C.P., Logato, P.V.R. & Moraes, A.M. 2006. Production and characterisation of alginate microparticles incorporating Aeromonas hydrophila designed for fish oral vaccination. Process Biochemistry, 41: 638–643.
Saberi, N. 2012. Optimization of Bacillus thuringiensis production in laboratory Fermenter. M.sc. thesis, Islamic Azad University, Sciences, and Research Branch of Tehran.
Salamouny, S.E.I., Ranwala, D., Shapiro, M., Shepard, B.M. & Farrar, R.R. 2009. Tea, coffee, and cocoa as ultraviolet radiation protectants for the beet armyworm nucleopolyhedrovirus. Journal of Economic Entomology, 102(5): 1767-1773.
Satinder, K., Verma, M., Tyagi, R.D. & Valero, J.R. 2006. Recent advances in downstream processing and formulations of Bacillus thuringiensis based biopesticides. Process Biochemistry, 41, 323–342.
Scher, H. 1997. Microencapsulated pesticides. Journal of Controlled Release Pesticides, 53: 126–144.
Villamizar, L., Barrera, G., Cotes, A.M., & Matinez, F. 2010. Eudragit S100 microparticles containing Spodoptera frugiperda nucleopolyehedrovirus: Physicochemical characterization, photostability and in vitro virus release. Journal of Microcapsulation, 27: 314–324.
 

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