Evaluation some polymer combinations in improvement of HaNPV stability against ultraviolet radiation

Document Type : Research Paper

Authors

Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

The current project was carried out to investigate the role of microencapsulation and influence of different polymers on the protection of Helicoverpa armigera nuclear polyhedrosis virus (HaNPV) against ultraviolet radiation.  For this purpose, microencapsulated formulation of HaNPV was produced by emulsion technique using three natural polymers including sodium alginate, gelatin, and starch individually in and combined with each other. After exposing to UV irradiation for 5 and 24 hours, the stability and viral activity of the obtained formulations were evaluated by bioassay test on H. armigera larvae. However, the mortality rate for the non-microencapsulated virus suspension, declined from 100% before UV radiation to 46.66% after 24 hours exposing to the UVA radiation. Before UV radiation, each of the sodium alginate and starch microencapsulated formulations separately, not only could provide a proper coating for HaNPV virus, but also decreased the viral activity. Whereas the combination of these two polymers together had a synergistic effect and resulted in an increased mortality. After 5 and 24 hours exposing to UV, their combination was significantly different from the non-microencapsulated formulation (Duncan test, P<0.05). After 5 and 24 hours exposing to UV, the difference of gelatin and its combinations with the non-microencapsulated formulation was significant.

 

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References

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