Antagonistic effects of Fusarium spp. associated with females and egg masses on Meloidogyne javanica In in vitro conditions

Document Type : Research Paper

Authors

1 Department of Plant Protection, College of Agriculture, University of Kordestan, Iran

2 Agricultural and Natural Resources Research Center of Kermanshah, Iran

3 Deppartmetn of Plant Protection, College of agriculture, University of Razi, Kermanshah, Iran

Abstract

In order to investigate the antagonistic effects of the fungi associated with adult females and egg masses of Meloidogyne javanica in tomato and cucumber grown in greenhouses of Kermanshah province, research experiments were performed in 2009-2010. Among isolated fungi there were 49 isolates of Fusarium spp. from which F. chlamydosporum, F. oxysporum, F. proliferatum, F. solani, F. tricinctum and F. verticillioides were identified. For purification and propagation of the nematode, single egg masses of adult females were inoculated on the roots of cucumber seedlings in the growth chamber. After nematode propagation, pathogenicity experiments were carried out in transparent plastic trays with a completely randomized design. Antagonistic effects of these fungal species were investigated on M. javanica through parameters including parasitized eggs percent, hatching eggs percent and juvenile (J2) mortality. F. chlamydosporum, F. solani and F. oxysporum had the highest ability to parasitizing the eggs. F. solani for its effect on preventing egg hatching and all Fusariums isolates for their effects on juvenile mortality had significant difference. In conclusion, F. solani had the highest antagonistic effects over others and also had the most abundance.
 
Brown, R.H. & Kerry, B.R. 1987. Principles and Practices of Nematode Control in Crops. Academic press, NewYork, 447 pp.
Darvishnia, M., Alizadeh, A. & Mohammadi Goltapeh, E. 1998. fusarium species and other fungi associated with crown and root rot of wheat in Lorestan Province. 13th Iran. Plant Protection Congress, Karaj, P. 20.
Dhingra, O.D. & Sinclair, J.B. 1986. Basic Plant pathology Methods. CRS perss, 355 pp.
De Grisse, A.T. 1969. Redescription et modification de quelques techniques utilisées dans l'étude des nematodes phytoparasitaires. Mededelingen van de Rijksfa, Gent, 34: 351-369.
Ershad, D. 2009. Fungi of Iran. Agricultural Research, Education & Extension Organization. 531 pp.
Fatemy, S., Ahmadian-Yazdi, A., Parvizy, R., Ahmadi, A., Pakniat, M., Barooti, M., Askari, M. & Ershad, D. 1999. Fungal parasites of Heterodera schachtii in Iran. Pakistan Journal of Nematology, 17(1): 61-66.
Fatemy, S., Saeidi-Naeini, F. & Alizadeh, A. 2005.In vitro screening of fungi for parasitism against sugar beet cyst nematode  Heterodera schschtii. Nematologia Mediterranea, 33: 185-190.
Gerlach, W. & Nirenberg, H. 1982.The Genus Fusarium a Pictorial Atlas. Paul Parey, Berlin and Hamburg. Kommissionsverlag. 406 pp.
Godoy, G., Rodriguez-Kabana, R. & Morgan-Jones, G. 1983. Fungal parasites of Meloidogyne arenaria eggs in an Alabama soil. Nematropica, 13: 201–213.
Heidarian, A., Karimi Roozbehani, A.R. & Ershad, D. 2000. The fungal diseases of potato in Chahar Mahal Province. 14th Iran. Plant Protection Congress, Isfahan, P. 306.
Hussey, R.S. & Barker, K.R. 1973. A comparison of methods of collecting inocula of Meloidogyne spp. including a new technique. Plant Disease Reporter, 57: 1025-1028.
Ibrahim G.H., Al-Rehiayani, S.M. & Bellal, M.M. 2009. Use of biocontrol fungi, Bacillus thuringiensis and organic soil amendment to control root-knot nematode (Meloidogyne incognita) in tomato and eggplant, Mansoura University Journal of Agricultural Sciences, 34 (11): 10761–10770.
Khezrinejad, N., Ghosta, Y. & Niknam, Gh. 2006. Fungi associated with sugar beet cyst nematode from fields of W. Azarbaijan. Rostaniha, 7(2): 149-161. (In Persian with English summary).
Leslie, J.F. & Summerell, B.A. 2006. The Fusarium Laboratory Manual. Blackwell Publishing Asia, First edition, 388 pp.
Morgan-Jones, G., White, J.F. & Rodrigues-Kabana, R. 1984. Fungal parasites of Meloidogyne incognita in an Alabama soybean field soil. Nematropica, 14: 93-96.
Nelson, P.E., Tousson, T.A. & Marasas, W.F. O. 1983. Fusarium Species, an Illustrated Manual for Identification. The Pennsylvania State University Press, University Park, 193 pp.
Nickle, W.R. 1991. Manual of Agricultural Nematology. Marcel Dekker, New York, 1035 pp.
Rizvi, M.N. & Shameel, M. 2006. In vitro nematicidal activities of sea weed extracts from Karachi coast. Pakistan Journal of Botany, 38(4): 1245-1248.
Roohibakhsh, A. & Ershad, D. 1995. Isolation of six Fusarium species from citrus leaf spots in Western region of Mazandaran Province. 12th Iranian Plant Protection Congress, Rasht, P. 234.
Ruanpanun, P., Tangchitsomkid, N., Hyde, K.D. & Lumyong, S., 2010. Actinomycetes and fungi isolated from plant-parasitic nematode infested soils: screening of the effective biocontrol potential, indole-3-acetic acid and siderophore production. World Journal of Microbiology and Biotechnology, 26: 1569–1578.
Sankaranarayanan, C., Hussaini, S.S., Kumar, P.S. & Rangeswaran, R. 2002. Parasitism of Meloidogyne incognita eggs by Fusarium oxysporum and other fungi. Indian Journal of Nematology, 32 (1): 33-36.
Sharifi, K., Zare, R., Zamanizadeh, H. & Karimi Roozbehani, A.R. 2004. Investigation on the role of Fusarium solani in dry rot of potato tubers. 16th Iranian Plant Protection Congress, Tabriz. P. 208.
Singh, S. & Mathur, N. 2010. In vitro studies of antagonistic fungi against the root-knot nematode, Meloidogyne incognita. Biocontrol Science and Technology, 20(3): 275-282.
Stirling, G.R. & Mankau, R. 1977. Parasitism of Meloidogyne eggs by a new fungal parasite. Journal of Nematology, 10(3): 236-240.
Sun, M.H., Gao, L., Shi, Y.X., Li, B.J. & Lio, X.Z. 2006. Fungi and actinomycetes associated with Meloidogyne spp. eggs and females in China and their biocontrol potential. Journal of Invertebrate Pathology, 93: 22-28.
Zareen, A., Siddiqui, I.A. & Javad Zaki, M. 2000. Fungal parasites of root-knot nematodes. Pakistan Journal of Biological Science, 3(31): 478-480.

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References

Ahmadi, A.R., Sharifi-tehrani, A., Kheiri, A. & Hejaroude, Gh.A. 1998. Isolation of Paecilomyces spp. and Fusarium solani from Heterodera schachtii and their efficacy in biocontrol of the nematode eggs in vitro. Iranian Journal of Plant Pathology, 34(3,4): 56-59. (In Persian with English summary).
Barooti, S. 1987. A list of plant pathogenic nematodes of Iran up to 1986. Plant Pest and Diseases Research Institute of Iran, 40pp (In Persian).
Booth, C. 1971. The Genus Fusarium. Commonwealth Mycological Institiue, 237 pp.
Brown, R.H. & Kerry, B.R. 1987. Principles and Practices of Nematode Control in Crops. Academic press, NewYork, 447 pp.
Darvishnia, M., Alizadeh, A. & Mohammadi Goltapeh, E. 1998. fusarium species and other fungi associated with crown and root rot of wheat in Lorestan Province. 13th Iran. Plant Protection Congress, Karaj, P. 20.
Dhingra, O.D. & Sinclair, J.B. 1986. Basic Plant pathology Methods. CRS perss, 355 pp.
De Grisse, A.T. 1969. Redescription et modification de quelques techniques utilisées dans l'étude des nematodes phytoparasitaires. Mededelingen van de Rijksfa, Gent, 34: 351-369.
Ershad, D. 2009. Fungi of Iran. Agricultural Research, Education & Extension Organization. 531 pp.
Fatemy, S., Ahmadian-Yazdi, A., Parvizy, R., Ahmadi, A., Pakniat, M., Barooti, M., Askari, M. & Ershad, D. 1999. Fungal parasites of Heterodera schachtii in Iran. Pakistan Journal of Nematology, 17(1): 61-66.
Fatemy, S., Saeidi-Naeini, F. & Alizadeh, A. 2005. In vitro screening of fungi for parasitism against sugar beet cyst nematode  Heterodera schschtii. Nematologia Mediterranea, 33: 185-190.
Gerlach, W. & Nirenberg, H. 1982. The Genus Fusarium a Pictorial Atlas. Paul Parey, Berlin and Hamburg. Kommissionsverlag. 406 pp.
Godoy, G., Rodriguez-Kabana, R. & Morgan-Jones, G. 1983. Fungal parasites of Meloidogyne arenaria eggs in an Alabama soil. Nematropica, 13: 201–213.
Heidarian, A., Karimi Roozbehani, A.R. & Ershad, D. 2000. The fungal diseases of potato in Chahar Mahal Province. 14th Iran. Plant Protection Congress, Isfahan, P. 306.
Hussey, R.S. & Barker, K.R. 1973. A comparison of methods of collecting inocula of Meloidogyne spp. including a new technique. Plant Disease Reporter, 57: 1025-1028.
Ibrahim G.H., Al-Rehiayani, S.M. & Bellal, M.M. 2009. Use of biocontrol fungi, Bacillus thuringiensis and organic soil amendment to control root-knot nematode (Meloidogyne incognita) in tomato and eggplant, Mansoura University Journal of Agricultural Sciences, 34 (11): 10761–10770.
Khezrinejad, N., Ghosta, Y. & Niknam, Gh. 2006. Fungi associated with sugar beet cyst nematode from fields of W. Azarbaijan. Rostaniha, 7(2): 149-161. (In Persian with English summary).
Leslie, J.F. & Summerell, B.A. 2006. The Fusarium Laboratory Manual. Blackwell Publishing Asia, First edition, 388 pp.
Morgan-Jones, G., White, J.F. & Rodrigues-Kabana, R. 1984. Fungal parasites of Meloidogyne incognita in an Alabama soybean field soil. Nematropica, 14: 93-96.
Nelson, P.E., Tousson, T.A. & Marasas, W.F. O. 1983. Fusarium Species, an Illustrated Manual for Identification. The Pennsylvania State University Press, University Park, 193 pp.
Nickle, W.R. 1991. Manual of Agricultural Nematology. Marcel Dekker, New York, 1035 pp.
Rizvi, M.N. & Shameel, M. 2006. In vitro nematicidal activities of sea weed extracts from Karachi coast. Pakistan Journal of Botany, 38(4): 1245-1248.
Roohibakhsh, A. & Ershad, D. 1995. Isolation of six Fusarium species from citrus leaf spots in Western region of Mazandaran Province. 12th Iranian Plant Protection Congress, Rasht, P. 234.
Ruanpanun, P., Tangchitsomkid, N., Hyde, K.D. & Lumyong, S., 2010. Actinomycetes and fungi isolated from plant-parasitic nematode infested soils: screening of the effective biocontrol potential, indole-3-acetic acid and siderophore production. World Journal of Microbiology and Biotechnology, 26: 1569–1578.
Sankaranarayanan, C., Hussaini, S.S., Kumar, P.S. & Rangeswaran, R. 2002. Parasitism of Meloidogyne incognita eggs by Fusarium oxysporum and other fungi. Indian Journal of Nematology, 32 (1): 33-36.
Sharifi, K., Zare, R., Zamanizadeh, H. & Karimi Roozbehani, A.R. 2004. Investigation on the role of Fusarium solani in dry rot of potato tubers. 16th Iranian Plant Protection Congress, Tabriz. P. 208.
Singh, S. & Mathur, N. 2010. In vitro studies of antagonistic fungi against the root-knot nematode, Meloidogyne incognita. Biocontrol Science and Technology, 20(3): 275-282.
Stirling, G.R. & Mankau, R. 1977. Parasitism of Meloidogyne eggs by a new fungal parasite. Journal of Nematology, 10(3): 236-240.
Sun, M.H., Gao, L., Shi, Y.X., Li, B.J. & Lio, X.Z. 2006. Fungi and actinomycetes associated with Meloidogyne spp. eggs and females in China and their biocontrol potential. Journal of Invertebrate Pathology, 93: 22-28.
Zareen, A., Siddiqui, I.A. & Javad Zaki, M. 2000. Fungal parasites of root-knot nematodes. Pakistan Journal of Biological Science, 3(31): 478-480.

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