Biological and biochemical characterization of Pseudomonas fluorescens UTPF5 as a plant probiotic bacterium in Iran

Document Type : Research Paper

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Abstract

Finding new strains of plant probiotic bacteria is an important step in commercialization process. In this study, the biological and biochemical features of Pseudomonas fluorescens UTPF5 is evaluated as an important bacterium in Iran. Production of several metabolites such as auxin, hydrogen cyanide, siderophore and DAPG, as well as biofilm formation and regulator systems was studied. In addition, inhibition of growth Rhizoctonia solani was determined. Results showed that strain UTPF5 produced all mentioned biocontrol-related metabolites except phenazin. Addition of Fe2+ reduced the production of siderophore but increased hydrogen cyanide. The strain UTPF5 produced Gac/Rsm signal and increased hcnA and RsmZ expression. It produced no AHL signal. Biocontrol potential and plant growth promotion in sunflower, millet, canola and bean was described by application of the bacterium in greenhouse trials.

 

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