Phylogenetic classification of Helicoverpa armigera Nucleopolyhedrovirus (HaNPV), isolated from Tobacco fields in the north of Iran, using RAPD markers

Document Type : Short Article

Abstract

For screening Helicoverpa armigera Nucleopolyhedrovirus (HaNPV) isolated from tobacco fileds of Mazandaran and Golestan Provinces in northern Iran, out of 10 Operon RAPD primers (10-mer) that randomly selected, two primers (OPM-03 and OPM-16) which showed the highest polymorphism were selected. Phylogenetic analysis was done using PyElph software and UPGMA method. Then, phylogenetic tree and genetic distances table were drawn. The cluster analysis divides the 16 HaNPV isolates into five distinct groups.  Such screening of HaNPV isolates is useful for improving biological control measures against tobacco budworm and cotton bollworm on different crops.

 

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References

Assemi, H., Rezapanah, M., Vafai-Shoushtari, R. & Mehrvar, A. 2012. Modified artificial diet for rearing of tobacco budworm, Helicoverpa armigera using the Taguchi method and Derringer’s desirability function. Journal of Insect Science, doi: 10.1673/031.012.10001.
Assemi, H., Sajjadi, S.A., Rezapanah, M., Mehrvar, A., Shahadati-Moghadam, Z. & Shazdeh-Ahmadi, N. 2013. Genetic diversity of isolates Helicoverpa armigera Nucleopolyhedrovirus on tobacco budworm using PCR-RFLP technique. Full papers of 8th national Biotechnology Congress in Iran and 4th national Biosafety Congress in Iran, Tehran, Iran, 6-8 July 2013.
Fakrudin, B., Prakash, S.H., Krishnareddy, K.B., Vijaykumar, Badari Prasad, P.R., Patil, B.V. & Kuruvinashetti, M.S. 2004. Genetic variation of cotton bollworm, Helicoverpa armigera (Hübner) of South Indian cotton ecosystem using RAPD markers. Current Science, 87: 1654-1659.
Geetha, N. & Rabindra, J. 2000. Genetic variability and comparative virulence of some geographic  isolates of  nuclear polyhedrosis virus of Helicoverpa armigera Hübner. pp: 65-80. In: Ignacimuthu, S., Sen, A. & Janarthanam, S. (Eds.), Biotechnological Application for Integrated Pest Management. Oxford and IBH Publication Company.
Getting, R.R. & McCarthy, W.J. 1982. Genotypic variation among wild isolates of Heliothis spp. nuclear polyhedrosis viruses from different geographical regions.Virology, 117: 242-245.
Jehle, J.A., Lange, M., Wang, H., Zhihong, H., Wang, Y. & Hauschild, R. 2006. Molecular identification and phylogenetic analysis of baculoviruses from Lepidoptera. Virology, 346: 180-193.
Khiaban, N.G.M.Z., Hejazi, M.S., Irani-Nejad, K.H., Mohammadi, S.A. & Khaghaninia, S. 2010. Genetic variability of geographical populations of the bollworm, Helicoverpa armigera Hübner (Lepidoptera: Noctuidae), in west and northwest of Iran. Munis Entomology & Zoology, 5 (2): 670-676.
Mehrvar, A., Rabindra, R.J., Veenakumari, K. & Narabenchi, G.B. 2008. Molecular and biological characteristics of some geographic isolates of nucleopolyhedroviruses of Helicoverpa  armigera (Lep., Noctuidae). Journal of Entomological Society of Iran, 28(1): 39-60.
Patel, C.S., Jani, J.J., Parekh, V. B., Darji, V. B., & Vaishnav, P. R. 2009. Genetic diversity and differentiation of Helicoverpa armigera nuclear polyhedrosis virus isolates from India. Phytoparasitica, 37(5), 407-413.
Sajjadi, A., Assemi, H. & Rezapanah, M. 2013. Genetic diversity of isolates Helicoverpa armigera Nucleopolyhedrovirus on tobacco budworm using PCR-RFLP technique. Abstracts of 4th International Participated Entomopathogens and Microbial Control, Artvin, Turkey. 11-14 September 2013. P: 75.
 
BioControl in Plant Protection
Volume 3, Issue 2 - Serial Number 2
March 2016
Pages 123-129

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