Effectiveness of the chemical stabilizers of fungal antagonist, Talaromyces flavus in biological control of tomato and greenhouse cucumber Rhizoctonia-induced seedling damping-off disease

Document Type : Research Paper

Authors

Scientific Board Member/ Iranian Research Institute of Plant Protection

BCPP-1602-84 (R1)

Abstract

Fungal antagonist, Talaromyces flavus, is one of the most important biocontrol agents of soil borne plant diseases including seedling damping-off. In this research, to increase the effectiveness of T. flavus isolates obtained from cucumber greenhouses and tomato fields, five chemical stabilizers were tested. Based on the results of the previous studies, the most effective substrate for the growth, sporulation and stability of T. flavus isolates related to the above-mentioned plants was a mixture of rice bran and peat-moss. For each plant, the greenhouse experiment was performed as randomized complete blocks with seven treatments and three replications. Experimental treatments included five different stabilizers (aminophenol, D- cycloserine, magnesium sulfate, carboxymethyl cellulose, and sodium nitrate), infected control and healthy control. The final evaluation of different treatments was based on the number of healthy plants. The overall results of this study, indicted that T. flavus inocula containing sodium nitrate, D- cycloserine, and carboxymethyl cellulose were more effective than aminophenol and magnesium sulfate in controlling seedling damping-off disease.

 

Keywords


Alimi, T., Ajewole, O.C., Olubode-Awosola, O.O. & Idowu, E.O. 2006. Economic rationale of commercial organic fertilizer technology in vegetable production in Osun State of Nigeria. Journal of Applied Horticulture, 8(2): 159-164.
Ardakani, S.S., Heydari, A., Khorasani, N. & Arjmandi, R. 2010. Development of new bioformulations of Pseudomonas fluorescens and evaluation of the products against damping-off of cotton seedlings. Journal of Plant Pathology, 2(1): 83-88.
Aziz, N.H., EL-Fouly, M.Z., EL- Essawy, A.A. & Khalaf, M.A. 1997.  Influence of bean seedling root exudates on the rhizosphere colonization by Trichoderma lignorum for the control of Rhizoctonia  solani. Botany  Bulletin  Academic  Science, 38(1): 33-39.
Budge, S.P. & Whipps, J.M. 2001. Potential for integrated control of Sclerotinia sclerotiorum in glasshouse lettuce using Coniothyrium minituns and reduced fungicide application. Phytopathology, 91(2): 221-227.
Caramez, M., Damaso, T., Costaterzi, S., Farias, A.X., Pereira de Oliveira, A.C., Fraga, M.E. & Couri, S. 2012. Selection of cellulolytic fungi isolated from diverse substrates. Brazilian Archives of Biology and Technology, 55(4): 513-520.
Christen, A.A. 1982. A selective medium for isolating Verticillium albo-atrum from soil. Phytopathology, 72(1): 47-49.
Cimarelli, C., Pulmieri, G. & Volpini, E. 2001. Ready N-alkylation of enantiopure aminophenols: synthesis of tertiary aminophenols. Tetrahedron, 57(28): 6089-6096.
Duo-Chuan, L.I., Chen, S. & Jing, L. 2005. Purification and partial characterization of two chitinases from the mycoparasitic fungus Talaromyces  flavus. Mycopathologia, 159(2): 223-229.
Engelelkes, C.A., Nuclo, R. L. & Fravel, D.R. 1997. Effect of Carbon, Nitrogen, and C:N ratio on growth, sporulation and biocontrol efficacy of Talaromyces flavus. Phytopathology, 87(1): 500-505. 
Ghaderi-Daneshmand, N., Bakhshandeh, A. & Rostami, M.R. 2012. Biofertilizer affects yield and yield components of wheat. International Journal of Agriculture Research and Review, 2: 699-704.
Gonzales Garcia, V., Purtal Onco, M.A. & Rubio Susan, V. 2006. Review, biology and systematics of the form genus Rhizoctonia. Spanish Journal of Agriculture Research, 4(1): 55-79.
Hadar, E. & Katan, J. 1989. The use of nitrate non utilizing mutants and a selective medium for studies of pathogenic strains of Fusarium oxysporum. Plant Disease, 73: 800-803.
Haggag, W.M., Kansoh, A.L. & Aly, A.M. 2006. Proteases from Talaromyces flavus and Trichoderma harzianum: Purification characterization and antifungal activity against brown spot disease on faba bean. Plant Pathology Bulletin, 15: 231-239.
Hiscox, J., Savoury, M., Vaughan, L.P., Muller, C.T. & Boddy, L. 2015. Antagonistic fungi interactions influence carbon dioxide evolution from decomposing wood. Fungal Ecology, 14: 24-32.
Husen, E., Simanungkalit, R.D.M., Suraswati, R. & Irawan, I. 2007. Characterization and quality assessment of Indonesian commercial biofertilizer. Indonesian Journal of Agricultural Science, 8: 31-38.
Inbar, J. & Chet, I. 1995. The role of  recognition in the induction of specific chitinases during mycoparasitism by Trichoderma harzianum. Microbiology, 141: 2823-2829.
Jat, G. & Agalave, H.R. 2013. Antagonistic properties of trichoderma species against oilseed- borne fungi. Science Research Reporter, 3: 171-174.
Kaewchai, S., Soytong, K. & Hyde, K.D. 2009. Mycofungicides and fungal biofertilizers. Fungal Diversity, 38(1): 25-50.
Katan, A.J. & Ovadia, S. 1975. An improved selective medium for the isolation of Verticillium dahliae. Phytoparasitica, 3(2): 133-137.
Keszler, A., Forgacs, E. & Kotai, L. 2000. Deparation and identification of volatile components in the fermentation broth of Trichoderma atroviride by solid-phase extraction and gas chromatography-mass spectrometry. Journal of Chromatographic Science, 38(1): 421-424.
Kim, K.K., Fravel, D.R. & Papavizas, G.C. 1989. Identification of a metabolite produced by Talaromyces flavus as glucose oxidase and its role in the biocontrol of Verticillium dahliae. Phytopathology, 78(4): 488-492.
Koch, E. 1999. Evaluation of commercial products for microbial control of soil-borne plant disease. Crop Protection, 18(2): 119-125.
Lee, S. & Lee, J. 2009. Color stabilization of low toxic antimicrobial polypropylene/poly (hexamethylene guanidine) phosphate blends by Taguchi technique. Macromolecular Research, 17(1): 411-416.
Madi, L., Katan, J. & Henis, Y. 1992. Inheritance of antagonistic properties and lytic enzyme activities in sexual crosses of Talaromyces flavus. Annual Applied Biology, 121(3): 565-576.
Madi, L., Katan, T. & Katan, J. 1997. Biological control of Sclerotium rolfsii and  Verticillium dahliae by Talaromyces flavus is mediated by different mechanisms. Phytopathology, 87(10): 1051-1060.
Matos, M., Simpson, B.K., Ramierz, H.L., Cao, R., Torres-Labandeira, J.J. & Hernandez, K. 2012. Stabilization of glucose oxidase with cyclodextrin- branched carboxymethylcellulose. Biotechnologia Aplicada, 29(1): 1-6.
Menz, G., Aldred, P. & Vriesekoop, F. 2011. Growth and survival of food borne pathogens in beer. Journal of Food Protection, 74: 1670-1675.
Merwel, C., Hansen, B.S., Maurice, H. & Vaughan, J.R. 1974. Mechanism of action of the mycotoxin Trichodermin, a 12,13-Epoxytrichothecene. Proceedings of the National Academy of Sciences of the united states of America, 71: 713-717.
Mikhail, M.S., Sabet, K.K., Omar, M.R., Hussein, E.M. & Kasem, K.K. 2009. Pathogenicity and protein electrophoresis of different cotton Rhizoctonia solani isolates. Egyptian Journal of Phytopathology, 37: 21-33.
Mukhopadhyay, S. & Maiti, S. K. 2009. Biofertilizer: VAM fungi- A future prospect for biological reclamation of mine degraded lands. Indian Journal Environmental Protection, 29: 801-808.
Naraghi, L., Heydari, A., Rezaee, S., Razavi, M., Jahanifar, H. & Mahmoodi Khaledi, E. 2010. Biological control of tomato Verticillium wilt disease by Talaromyces flavus. Journal of Plant Protection Research, 50: 360-365.
Naraghi, L., Heydari, A., Rezaee, S. & Razavi, M. 2012. Biocontrol agent Talaromyces flavus stimulates the growth of cotton and potato. Journal of Plant Growth Regulation, 31: 471-477.
Naraghi, L., Heydari, A., Rezaee, S. & Razavi, M. 2013. The study of mechanisms of antagonism against Verticillium dahliae and Talaromyces flavus fungus Verticillium albo-atrum causes wilt disease in several important crops. Biocontrol in Plant Protection, 1(1): 13-28. (In Persian with English Summary).
Pascual, S., Melgarejo, P& . Magan, N. 1999. Production of the fungal biocontrol agent Epicoccum nigrum by solid substrate fermentation: effect of water activity on accumulation of compatible solutes. Mycopathologia, 146(2): 83-89.
Patil, N.S. & Jadhav, J.P. 2015. Penicillium ochrochloron MTCC 517 chitinase: An effective tool in commercial enzyme cocktail for production and regeneration of protoplasts from various fungi. Saudi Journal of Biological Sciences, 22(2): 232-236.
Pereira, I., Ortegu, R., Barrientus, L., Moya, M., Reyes, G. & Kramm, V. 2009. Development of a biofertilizer based on filamentous nitrogen- fixing cyanobacteria for rice crops in Chile. Journal of Applied Phycology, 21(1): 135-414.
Sargin, S., Gezgin, Y., Eltem, R. & Vardar, F. 2013. Micropropagule production from Trichoderma harzianum EGE-K38 using solid-state fermentation and a comparative study for drying methods. Turkish Journal of Biology, 37(1): 139-146.
Schuster, A.&  Schmoll, M. 2010. Biology and biotechnology of Trichoderma. Applied Microbiology and Biotechnology, 87(3): 787-799.
Shakhul Ashraf, M. & Ahmad Khan, T. 2007. Efficacy of Gliocladium virens and Talaromyces flavus with and without organic amendments against  Meloidogyne javanica infecting eggplant. Asian Journal of Plant Pathology, 1(1): 18-21.
Shanmugam, V., Ronen, M., Shalaby, S., Larkov, O., Rachamim, Y., Hader, R., Rose, M., Carmeli, S., Horwitz, B.A. & Leu, S. 2010. The fungal pathogen Cochliobolus heterostrophus responds to maize phenolics: novel small molecula signals in a plant-fungal interaction. Celluar Microbiology, 12: 1421-1431.
Sharzehei, A., Heidary, S. & Raufi, F. 2011. Identification of tomato root and crown pathogenic fungi in Marvdasht region, Iran. Quarterly Journal of Research in Plant Pathology, 1: 57-65.
Sneh, B., Burpee, L. & Ogoshi, A. 1991. Identification of Rhizoctonia species. APS press.
Sneh, B., Yamoah, G. & Stewart, A. 2004. Hypovirulent Rhizoctonia spp. isolates from New Zealand soils protect radish seedlings against damping-off caused by Rhizoctonia solani. New Zealand Plant Protection, 57(1): 54-58.
Vasane, S.R. & Kothari, R.M. 2008. An integrated approach to primary and secondary hardening of banana Var. Grand Naine. Indian Journal of Biotechnology, 7(2): 240-245.
Veverka, K., Stolcova, J. & Ruzek, P. 2007. Sensitivity of fungi to urea, ammonium nitrate and their equimolar solution UAN. Plant Protection Science, 43:4: 157-164.
Vincelli, P.C. & Beaupre, C.M.S. 1989. Comparison of media for isolating Rhizoctonia solani from soil. Plant Disease, 73(12): 1014-1017.
 Ward, O.P. 2011. Production of recombinant proteins by filamentous. Biotechnology Advances, 30(5): 1112-1139.
Yu, M.Y. & Chang, S.T. 1987. Effects of osmotic stabilizers on the activities of mycolytic enzymes used in fungal protoplast liberation. World Journal of Microbiology and Biotechnology, 3(2): 161-167.
Zamanizadeh, H.R., Hatami, N., Aminaee, M.M. & Rakhshandehroo, F. 2011. Application of biofungicides in control of damping disease off in greenhouse crops as a possible substitute to synthetic fungicides. International Journal of Environmental Science and Technology, 8(1): 129-136.