Effect of some herbal compounds on fungal and bacterial infections in micropropagation of plants

Document Type : Short Article

Authors

Assistant prof. of Plant Protection Dept. of University of Mohaghegh Ardabili

BCPP-1503-22 (R5)

Abstract

Fungal and bacterial infections are main problems in the micropropagation of bulbous plants which can affect the efficiency of these propagation methods.To investigate the effect of different concentrations of herbal compounds on the growth and regeneration of crown imperial, an experiment was conducted based on the completely randomized design in factorial arrangement with five replications. Treatments included various concentrations of S-carvon, thymol and carvacrol. The concentrations of thymol and carvacrol significantly (P<0.05) inhibited the fungal and bacterial growth. Thymol at 150 ppm and carvacrol at 100 ppm completely inhibited the growth of Alternaria sp. (100%). Investigation on the effects of plant extract compounds on bacterial pathogens removal revealed that 200 ppm of thymol and carvacrol completely inhibited the growth of Pectobacteriumcarotovora subsp. carotovorum with 0.1-0.2 OD (Optical density). Moreover, maximum growth inhibition of P.carotovorum subsp. carotovorum with OD=2 by thymol and carvacrol was observed at the rate of 300 and 400 ppm, respectively. Various concentrations of S-carvon did not significantly affect fungal and bacterial pathogens comparing to the control. In general, carvacrol and thymol could effectively prevent the fungal and bacterial growth and could be used as a potential candidate for the prevention of fungal and bacterial infection of culture medium in the micropropagation of plants such as crown imperial.

Keywords

References

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BioControl in Plant Protection
Volume 4, Issue 1 - Serial Number 7
January 2017
Pages 79-84

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