Evaluation of the efficacy of Trichoderma isolates in the biological control of soybean charcoal rot disease in the laboratory and greenhouse conditions

Document Type : Research Paper

Authors

oil seeds research and development com.

Abstract

Soybean is considered as the most important oilseed crop in the world. One of the common diseases of soybean is charcoal rot which is caused by Macrophomina phaseolina. Chemical control of this disease is not effective and its integrated management using different methods is important. In this research study, in order to obtain suitable antagonist isolates for the disease biological control, effect of 126 Trichoderma isolates on the  causal pathogen, M. phaseolina was studied using dual culture and the effect of volatile compounds tests in the laboratory and 12 most effective isolates were selected for the greenhouse studies. Identification of the  isolates was done based on the morphological characteristics using valid keys showed that three isolates recognized as Trichoderma reesei, seven as T. harzianum and two as T. atroviride. Effects of the above-mentioned isolates with two control treatments (positive and negative) were investigated on soybean charcoal rot in the greenhouse. After soil pasteurization in the pots, pathogen was added and Trichoderma isolates were used at soybean planting time. In order to create suitable conditions for the pathogen infection, drought stress applied in the pots at early flowering. At the end of infection period, decreasing the disease incidence on soybean and decreasing the population of pathogen microsclerotia in the soil were observed and there were significant differences among different treatments (P<0.01) regarding both factors. The highest decrease of disease incidence and microsclerotia population were observed in the negative control (without Macrophomina and Trichoderma). Isolates ARCTr144 (T. reesei), ARCTr102 (T. harzianum), ARCTr184 (T. reesei) and ARCTr105 (T. atroviride) showing the highest decrease of disease incidence (62-75 percent) and microsclerotia population (73-85 percent) after the negative control were the best and most effective isolates.

 

Keywords

References

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BioControl in Plant Protection
Volume 5, Issue 1
September 2017
Pages 71-80

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