Effects of Pseudomonas fluorescens on the disease severity, physiological, and biochemical traits in the tubers of potato cultivars infected with Rhizoctonia solani under greenhouse conditions

Document Type : Research Paper

Authors

1 Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

2 College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

Abstract

To evaluate the effects of Pseudomonas fluorescens on potato production in the presence of Rhizoctoniasolani,a three replicated greenhouse experiment was conducted in a completely randomized factorial design containing  three strains of Pseudomonas fluorescens includingUTPF5(P5),P. fluorescensUTPF68 (P68) and P. fluorescens UTPF74(P74), Rhizoctonia solani AG3 and Agria and Sante cultivars of potato provided by the seed and plant certification and registration institute. Disease severity, root dry weight, stolon length and dry weight, tubers number, dry weight and fresh weight, Antioxidant activity (GPX, β-1, 3-glucanase), proline content, MDA and soluble sugar content were determined in different treatments. The results showed that Pseudomonas fluorescens treatment had a significant effect on all traits in the presence of fungus(pathogen-infected condition). The fungal virulence was also significantly decreased in this treatment compared to the control. P5 and Santevariety combined treatment showed highly significant effect on the measured traits than the other combined treatments, so that the highest amounts of dry weight of root, stolon length and dry weight, tubers dry and fresh weight and the number of stolon were observed in this treatment with 0.62, 0.86, 1.40, 2.31, 1.37 and 0.63 folds increase respectively compared to the control. Results also indicated that in P5 treatment Antioxidant enzyme activity, (GPX, β-1, 3-glucanase), proline and soluble sugar content were increased by 1.32, 0.61, 2 and 2.6 and folds respectively and lipid peroxidation was decreased by 53% in comparison with the control. 

Keywords

References

Ahmadzadeh, M. 2014. Biological Control of Plant Diseases Plant Probiotic Bacteria. 2nd ed. University of Tehran (In Persian).
Anonymous, 2017. Crop statistics. Office of static and information technology, assistant planning and economy of Ministry of Agriculture.
Antoun, H. & Kloepper, J.W. 2001. Plant growth-promoting rhizobacteria (PGPR), In: Brenner, S. & Miller, J.H., (eds.), Encyclopedia of Genetics. Academic Press, N.Y.
 
Arabiat, S.I. & Khan, M. 2014. Sensitivity of Rhizoctonia solani to Fungicides. Oral Technical Session: Disease Control and Pest Management. 2014. APS-CPS Joint Meeting, August 9-13, Minneapolis, Minnesota, USA.
Atkinson, D., Thornton, M.K. & Miller, J.S. 2011. Development of Rhizoctonia solani on stems, solon and tubers of Potato II. Efficacy of chemical applications. American Journal of Potato Research, 88: 96–103.
Bates, L.S. Waldren, R.P. & Teare, L.D. 1973. Rapid determination of free proline for water-stress tudies. Plant and Soil, 39: 205–207.
Behdad, A. 2010. Plant Disease. Publication Neshat of the University of Isfahan (In Persian).
Brewer, M.T. & Larkin, R.P. 2005. Efficacy of several potential biocontrol organisms against Rhizoctonia solani on potato. Crop Protection, 24: 939–950.
Brierley, J.L., Hilton, A. J., Wale, S.J., Woodhall, J.W. & Lees, A.K. 2016. The Relative importance of seed- and soil-borne inoculum of Rhizoctonia solani AG-3 in causing black scurf on Potato. Potato Research, 59:181–193.
Cecchini,N.M., Monteoliva, M.I. & Alvarez, M.E. 2011. Proline dehydrogenase contributes to pathogen defense in Arabidopsis. Plant Physiol, 155: 1947–1959.
Dionisio-Sese, M.L. & Tobita, S. 1998. Antioxidant responses of Rice seedlings to salinity stress. Plant Science, 135: 1–9.
Du, Z. & Bramlage, W.J. 1992. Modified thiobarbituric acid assay for measuring lipid oxidation in sugar-rich plant tissue extracts. Journal of Agricaltural Food Chemestry, 40: 1566–1570.
Elkahoui, S., Dje´bali, N., Yaich, N., Azaiez, S.,  Hammami, M., Essid, R. & Limam F. 2015. Antifungal activity of volatile compounds-producing Pseudomonas P2 strain against Rhizoctonia solani. World Journal Microbiology Biotechnology, 31: 175–185.
Gutierrez-Manero, F.J., Ramos-Solano, B., Probanza, A., Mechouachi, J., Tadeo, F.R. & Talon, M. 2001. The plant-growth-promoting rhizobacteria Bacillus pumilus and Bacillus licheniformis produce high amount of physiologically active gibberellins. Physiology Plant, 111: 206–211.
Haas, D. & Geneviève, D. 2005. Biological control of soil-borne pathogens by Pseudomonas fluorescent. Nature Reviews Microbiology, 3: 307–19.
Heath, R.L. & Packer, L. 1968. Photoperoxidation in isolated chloroplasts: Kinetics an stoichiometry of fatty acid peroxidation. Archive Biochemistry Biophysics, 125: 189–198.
Hooker, W.J. 1983. Research for the potato in the year 2000. CIP Lima, Peru.
Khedher, S.B., , Kilani-Feki, O., Dammak, M., Jabnoun-Khiareddine, H., Daami-Remadi, M. & Tounsi, S. 2015. Efficacy of Bacillus subtilis V26 as a biological control agent against Rhizoctonia solani on potato. Plant biology and pathology, 338: 784–792.
Kumar, S.S., Krishna Rao, M.R., Deepak Kumar, R., Panwar, S. & Prasad, C.S. 2013. Biocontrol by plant growth promoting rhizobacteria against black scurf and stem canker disease of potato caused by Rhizoctonia solani. Archives of Phytopathology and Plant Protection, 46: 487–502.
Larkin, P.R. 2016. Impacts of biocontrol products on Rhizoctonia disease of potato and soil microbial communities, and their persistence in soil. Crop Protection, 90: 96–105.
Małolepsza, U., Nawrocka, J. & Szczech, M. 2017. Trichoderma virens 106 inoculation stimulates defence enzyme activities and enhances phenolic levels in tomato plants leading to lowered Rhizoctonia solani infection. Biocontrol science and technology, 27: 180–199.
Miller, G.L. 1959. Use of dinitrosalicylic acid reagent for the determination of reducing sugar. Analytical Biochemistry, 31: 426–428.
Patten, C.L. & Glick, B.R.. 2002. The role of bacterial indolacetic acid in the development of the host plant root system. Applied and Environmental Microbiology, 68: 3795–3801.
Pieterse, C.M.J., Zamioudis, C., Berendsen, R.L., Weller, D.M., VanWees, S.C.M. & Bakker, P.A.H.M. 2014. Induced systemic resistance by beneficial microbes. Annual Review of Phytopathology, 52: 347–75.
Piromyou, P.B. Buranabanyat, B., Tantasawat, Tittabutr, P. Boonkerd, N. & Teaumroong, N. 2011. Effect of plant growth promoting rhizobacteria (PGPR) inoculation on microbial community structure in rhizosphere of forage corn cultivated in Thailand. European Journal of Soil Biology, 47: 44–54.
Saikia, R., Pratab Singh, B., Kumar, R., and Arora, K. 2005. Detection of pathogenesis-related proteinschitinase and β-1,3-glucanasein induced chickpea. Current Science 89(4): 659–663.
Sheligl, H.Q. 1986. Die verwertung orgngischer souren durch chlorella lincht. Planta Journal, 47–51.
Shoresh, M. Harman, G.E. & Mastouri, F. 2010. Induced systemic resistance and plant responses to fungal biocontrol agents. The Annual Review Annual Review of Phytopathology, 48: 21–43.
Singh, S.P., Gupta, R., Gaur, R. & Srivastava, A.K. 2015.  Streptomyces spp. alleviate Rhizoctonia solani-mediated oxidative stress in Solanum lycopersicon. Annals of Applied Biology, 168: 232–242.
Taheri, P., Irannejad, A., Goldani, M. & Tarighi, S. 2014. Oxidative burst and enzymatic antioxidant systems in rice plants during interaction with Alternaria alternata. European Journal of Plant Pathology, 140: 829–839.
Vessey, J. K. 2003. Plant growth promoting rhizobacteria as biofertilizers. Plant Soil, 255: 571–586.
Weller, D. M. & Cook, R. J. 1983. Suppression of take-all of wheat by seed treatments with fluorescent Pseudomonas. Phytopathology, 78: 463–469.
Wilson, P.S., Ketola, E.O., Ahvenniemi, P.M., Lehtonen, M.J. & Valkonen, J.P.T. 2008. Dynamics of soilborne Rhizoctonia solani in the presence of  Trichoderma harzianum: effects on stem canker, black scurf and progeny tubers of potato. Plant Pathology, 57: 152–161. 
BioControl in Plant Protection
Volume 5, Issue 2 - Serial Number 10
December 2018
Pages 43-54

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