Solid-state fermentation and viability of Pochonia chlamydosporia and Purpureocillium lilacinum on some organic substrates

Document Type : Research Paper

Authors

1 Department of Plant Protection, Faculty of Agricultural Sciences and Food Industries, Science & Research Branch of Islamic Azad University, Tehran, Iran

2 Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extention Organization (AREEO), Tehran, Iran

Abstract

Pochonia chlamydosporia and Purpureocillium lilacinum are important biocontrol agents of root-knot and cyst nematodes. Both fungi were first reported from sugar beet cyst nematode from Khorasan province and have shown promising potential as biocontrol agents of Heterodera schachtii, Globodera rostochiensis and Meloidogyne spp. In this study, sporulation and shelf life of fungi were evaluated on solid substrates for 2 months. The fungi were grown on cereals, their spore production and stability at 10 °C and 25 °C were evaluated. Based on media, storage duration and temperature regime, spore production of fungi differed. Pochonia produced spores on wheat grain, barley grain, wheat and rice bran after 30 days, and on rice husk after 60 days. P. lilacinum colonizedall the substrates and yield of spore was highest on barley grain and rice husk. Viability of both fungi was slightly decreased after 60 days storage at 25 ºC, but at 10 ºC with some discrepancy did not change significantly. After 60 days, Pochonia produced average 1.5 × 108 and 7 × 107 spores / g of wheat and rice bran, and other cereals, respectively. Meanwhile, P. lilacinum produced 2.7 × 108 and 1.5 × 108 spores /g wheat and barley bran, respectively. Chlamydospores are resting spores of P. chlamydosporia var. chlamydosporia and one of the most common sources of inoculation in soil. On barley 1.1× 105 and on wheat and rice bran average 5 × 103 chlamydospores were produced.

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References

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