Biological control of root-knot nematode Meloidogyne incognita, using Trichoderma harzianum Iran2375c on cucumber

Document Type : Research Paper

Authors

Department of Plant pathology, Agricultural Faculty, University of Shahr-e-Kord, Shahr-e-Kord, Iran

Abstract

Biocontrol of nematodes is now a priority in order to reduce the harmful effects of chemicals, including human health and environmental pollution. A lot of research suggest effective performance of Trichoderma spp. in reducing the damage and inhibition of various diseases. In this study, we investigated the effect of Trichoderma harzianum Iran2375c culture extracts on second stage juvenile mortality and egg hatching at 24, 48 and 72 hours using the concentrations of 1: 1, 1:10 and 1:100. In greenhouse studies, four leaf seedlings were inoculated with a concentration of 106 spores/g and after 10 days of inoculation of the soil, 3000 eggs and juvenile (stage two) of Meloidogyne incognita nematode per Kilogram were inoculated to the root of seedling. Experiment was carried out in greenhouse conditions in completely randomized design with four treatments in ten replications. Two month after inoculation of nematode, plant growth indices and indices related to nematode were measured. The concentration of 1:1 was the most effective in second stage juvenile mortality and egg hatching. Based on the results of greenhouse studies, the growth and nematode indices of treatments showed a significant difference. The results of this study indicates the potential of T. harzianum Iran2375cin biological control of M. incognita and promotion of plant growth.

Keywords

References

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BioControl in Plant Protection
Volume 6, Issue 2 - Serial Number 12
February 2019
Pages 81-91

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