[Short Article] Evaluation of the effects of Bacillus thuringiensis var. kurstaki in combination with thiacloprid and diatomaceous earth on second and third instar larvae of the large white butterfly, Pieris brassicae (L.) (Lep.: Pieridae)

Document Type : Short Article

Authors

Department of Plant Protection, Faculty of Agriculture, Islamic Azad University, Mahabad Branch

Abstract

The present study was performed to evaluation of the toxicity effects of Bacillus thuringiensis var. kurstaki, thiacloprid and diatomaceous earth on 2nd and 3rd larval instars of large white butterfly (Pieris brassicae) in the single or combination form under laboratory conditions. The LC50 values by probit analysis resulting from the effects of different concentrations of B. thuringiensis, thiacloprid and diatomaceous earth at 48 and 72 hours on the 2nd and 3rd larval instars, respectively for B. thuringiensis (1593.5 & 991.8), (1800 & 1150.9) ppm, for thiocloprid (4933.3 & 2705.9), (5208.6 & 2878.3) ppm and for diatomaceous earth (340.9 & 216.6), (386 & 254.9) mg/L were obtained. The result showed that in both larval instars the mixing of factors had an increasing effect compared to the separate effects of each, so that the average mortality in 48 and 72 hours on 2nd and 3rd instars larvae, respectively, (70 & 76.7) and (66.7 & 73.4) were reported. Based on laboratory bioassays, mixing of thiocloprid, diatomaceous earth with B. thuringiensis for integrated pest management is recommended.

Keywords

References

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BioControl in Plant Protection
Volume 7, Issue 1 - Serial Number 13
September 2019
Pages 157-162

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