Investigation on the pathogenicity of some Iranian isolates of Beauveria bassiana on the mulberry whitefly Aleuroclava jasmini sensu lato

Document Type : Short Article

Authors

1 Department of Entomology, Faculty of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Varamin, Tehran, Iran

2 Iranian Research Institute of Plant Protection, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran

Abstract

Mulberry whitefly Aleuroclava jasmini sensu lato Takahashi 1932 as a sap feeder insect is one of the most serious pest in mulberry trees in Tehran province, Iran since 2016. The population of the species has increased due to lack of natural enemies in the new area.In this paper, virulence of four Iranian isolates of B. bassiana on second nymphal stage of A. jasmini sensu lato in five concentrations through spraying under laboratory conditions were investigated. The number of dead nymphs was recorded daily to 10 days. Mean mortality percentages were analyzed by AVONA under 95% of confidence level in SPSS. LC50 and LT50 were calculated by SAS. The final results from Probit test showed that the lowest LC50 produced by to two isolates, PTCC5197 and IRAN1359 as 1.3 × 105 conidia ml–1 indicated higher pathogenicity against A. jasmini sensu lato. The highest LC50 belonged to IRAN1228 with the value of 9.9 × 105 conidia ml–1. LT50 results showed that by increasing the isolates concentration, mean lethal time decreases. It can be concluded that the studied isolates had high level of pathogenicity on A. jasmini sensu lato nymphs and it can be applied through IPM programs, indeed their environmentally safety would be so valuable.

Keywords

References

Askary, H., Kouhestani, K., Zarrabi, M. & Baghdadi, A. 2014. Evaluation of pathogenicity of integrated use of Beauveria bassiana and Lecanicillium muscarium on Bemisia tabaci (Homoptera: Aleyrodidae). Biological Control of Pests and Plant Diseases, 3(1): 31–30. (In Persian with English summary)
Ekesi, S., Shah, P.A., Clark, S.J. & Pell, J.K. 2005. Conservation biological control with the fungal pathogen Pandora neoaphidis: implications of aphid species, host plant and predator foraging. Agriculture and Forest Entomology,7: 21–30.
Malekan, N. 2009. Effect of Lecanicillium muscarium, Beauveria bassiana and Imidacholopride on different nymphal stages of greenhouse whitefly Trialeurodes vaporariorum, separatedly and combined. MSc. Thesis, Isfahan University of Technology, Isfahan, Iran. (In Persian with English summary)
Malekan, N., Hatami, B., Ebadi, R., Akhavan. A. & Radjabi, R. 2015. Evaluation of entomopathogenic fungi Beauveria bassiana and Lecanicillium muscarium on different nymphal stages of greenhouse whitefly Trialeurodes vaporariorum in greenhouse conditions. Biharean Biologist,9(2): 108–112.
Manzari, S., Farrokhi, S., Shahbazvar, N. & Hosseini, R. 2018. Natural enemies of the whitefly Aleuroclava jasmini sensu lato (Aleyrodidae) in Tehran green space. Proceeding of the 23th. Iranian Plant Protection Congress, Gorgan University of Agricultural Sciences and Natural Resources. P: 924. (In Persian with English summary)
Manzari, S., Ahmadipour, R., Shabazvar, N. & Farrokhi, S. 2017. Pealius mori (Hem.: Aleyrodidae): A new whitefly species record for Iran. Journal of Entomological Society of Iran, 37(2): 289–291.
Mascarin, G.M., Kobori, N.N., Quintela, D. & Delalibera, I.Jr. 2013. The virulence of entomopathogenic fungi against Bemisi tabaci biotype B (Hemiptera: Aleyrodidae) and their conidial production using solid substrate fermentation. Biological Control,66: 209–218.
Ondiaka, S., Maniania, N., Nyamasyo, G. & Nderitu, J. 2008. Virulence of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae to sweat potato weevil Cylas puncticollis and effects on fecundity and egg viability. Annals of Applied Biology, 153: 41–48.
Quesada–Moraga, E., Maranhao, E.A.A., Valverde–Garcia, P. & Santiago–Alvarez, C. 2006. Selection of Beauveria bassiana isolate for control of the whiteflies Bemisia tabaci and Trialeurodes vaporariorum on the basis of their virulence, thermal requirements and toxicogenic activity. Biological Control, 36(3):274–287.
Rasekh, B. 2010. Distribution, hosts range and natural enemies of Jasmine Whitefly Aleuroclava jasmini (Takahashi) in the Fars Province, Iran. MSc. Thesis, Science and Research Branch, Islamic Azad University, Tehran, Iran, (In Persian with English summary)
Thungrabeab, M. & Tongma, S. 2007. Effect of entomopathogenic fungi, Beauveria bassiana (Balsam) and Metarhizuim pliae (Metsch) on non–target insects. . KMITL Science and Technology Journal, 7(S1): 8–12.
Touhidul Islam, M.D., Dzolkhifli, O., Latif, M.A. & Mahbub Morshed, M.D. 2011. The integrated use of entomopathogenic fungus, Beauveria bassiana with botanical insecticide, neem against Bemisia tabaci on eggplant. African Journal of Microbiology Research, 5(21):3409–3413. DOI: 10.5897/AJMR11.478
Zafar, J., Freed, S., Ali Khan, B. & Farooq, M. 2016. Effectiveness of Beauveria bassiana against cotton whitefly, Bemisia tabaci (Gennadius) (Aleyrodidae: Homoptera) on different host plants.Pakistan Journal of. Zoology, 48(1): 91–99.

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