Using of auxin signaling pathway to investigate the contrast between two different strains of Bacillus against Agrobacterium

Document Type : Research Paper

Authors

1 Department of Plant Disease Research, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension, Tehran, Iran

2 Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

Abstract

Crown gall is one of the most damaging bacterial diseases caused economic damages. Nowadays special attention has been focused on biological control of plant diseases as an alternative to chemical control in terms of the dangers of pesticides. There was no special research focused on the interaction between Bacillus and Agrobacterium until now. The objective of this study was understanding molecular mechanisms between B. subtilis as a biocontrol agent and A. tumefaciens. This information would provide us the opportunity for better management of the disease. In this study tobacco plants (Nicotiana tabacum), IBRC–M10701strains of A. tumefaciens, B. subtilis strain ATCC21332, and Bacillus subtilis OKB105 strain FKR3 were used. miRNAs have an important effect in the auxin signaling pathway and resistance induction. The expression level of two miRNAs, miR167 and miR393 was measured using Real–Time PCR; 1, 3 and 6 days after inoculations of A. tumifaciens and B. subtilis revealed significant differences with the control plants. The results of expression levels demonstrated FKR3 showed a better effect to ATCC21332 in controlling A. tumifaciens. Research showed a positive effect of Bacillus on Agrobacterium. This study indicates the possible use of biological controls such as B. subtillis in controlling of A. tumefaciens.

Keywords

References

Allen, O.N., & Holding, A.J. 1974. In R.E. Buchanan & N.E. Gibbons (Eds.), Bergey’s manual of determinative bacteriology. Baltimore: Williams and Wilkins, 8: 264–267.
Amani, B. 1966. Stem androot gall of grapevine. Iranian Journal of Plant Pathology. 3: 12–18.
Bartel, D.P. 2004. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 116: 281–297.
Dunoyer, P., Himber, C. & Voinnet, O. 2006. Induction, Suppression and requirement of RNA silencing pathways in virulent Agrobacterium tumefaciens infections. Nature genetics, 38: 258–263.
Garrett, C.M.E. 1972. Crown gall (Agrobacterium tumefaciens). Report of East Malling Research Station for, 134–135.
Gray, W.M., Kepinski, S., Rouse, D., Leyser, O. & Estelle, M. 2001. Auxin regulates SCFTIR1–dependent degradation of Aux/ IAA proteins. Nature, 414: 271–276.
Kasschau, K.D., Xie, Z., Allen, E. Llave, C., Chapman, E.J., Krizan, K.A. & Carrington, J.C. 2003. P1/HC–Pro, a viral suppressor of RNA silencing, interferes with Arabidopsis development and miRNA function. Developmental Cell, 4: 205–217.
Kennedy, B.W. & Alcorn, S.M. 1980. Estimates of U.S. crop losses to prokaryote plant pathogens. Plant Disease, 64: 674–676.
Khraiwesha, B., Zhua, J.K. & Zhuc, J. 2012. Role of miRNAs and siRNAs in biotic and abiotic stress responses of plants. Biochimica et Biophysica Acta, 1819, 2: 137–148.
Kloepper, J.W., Ryu, C.M. & Zhang, S.A. 2004. Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytopathology, 94: 1259–1266.
Mallory, A.C., Bartel, D.P. & Bartelet, B. 2005. MicroRNA–directed regulation of Arabidopsis AUXIN RESPONSE FACTOR17 is essential for proper development and modulates expression of early auxin response genes. Plant Cell, 17: 1360–1375.
Navarro, L., Dunoyer, P., Jay, F., Arnold, B., Dharmasiri, N., Estelle, M., Voinnet, O. & Jones, J.D.G. 2006. A Plant miRNA Contributes to Antibacterial Resistance by Repressing Auxin Signaling. Science, 312: 436.
Nazari, F., Safaie, N., Soltani, B.M., Shams–Bakhsh, M. & Sharifi, M. 2017. Bacillus subtilis affects miRNAs and flavanoids production in Agrobacterium–Tobacco interaction. Plant Physiology and Biochemistry, 118: 98–106.
Ongena, M., Jourdan, E., Adam, A., Paquot, M., Brans, A., Joris, B., Arpigny, J. & Thonart, P. 2007. Surfactin and fengycin lipopeptides of Bacillus subtilis as elicitors of induced systemic resistance in plants. Environmental Microbiology, 9: 1084–1090.
Peypoux á, F., Bonmatin á, J.M. & Wallach, J. 1999. Recent trends in the biochemistry of surfactin. Applied Microbiology and Biotechnology, 51: 553–563.
Pruss, G.J., Nester, E.W. & Vance, V. 2008. Infiltration with Agrobacterium tumefaciens Induces Host Defense and Development–Dependent Responses in the Infiltrated Zone. MPMI, 21, 12: 1528–1538.
Rohrazi, K. & Rahimian, H. 2010. Isolation and identification of Rhizobium radiobacter from walnut crown scab in Golestan province, 19th Plant Protection Congress, 426.
Ryu, C.M., Farag, M.A., Hu, C.H., Reddy, M.S., Kloepper, J.W. & Pare, P.W. 2004. Bacterial volatiles induce systemic resistance in Arabidopsis. Plant Physiolology, 134: 1017– 1026.
Severin, V. & Dejeu, L. 1994. Bolile úi dăunătorii viĠei de vie. Editura Ceres Bucuresti, 124.
Sobiczewski, P., Karczewski, J. & Berczynski, S. 1991. Biological control of crown gall Agrobacterium tumefaciens in Poland. Fruit Science Report, 18: 125–132.
Stein, T. 2005. Bacillus subtilis antibiotics: structures, syntheses and specific functions. Molecular and Microbiology, 56(4): 845–857.
Sunkar, R., Li, Y.F. & Jagadeeswaran, G. 2012. Functions of microRNAs in plant stress responses. Trends in Plant Science, 17(4): 196–203.
Yang, Y., Hammes, U.Z., Taylor, C.G., Schachtman, D.P. & Nielsen, E. 2006. Highaffinity auxin transport by the AUX1 influx carrier protein. Current Biology, 16: 1123–1127.

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