Evaluation of the effectiveness of some bacterial isolates in biological control of rice blast disease

Document Type : Research Paper

Authors

1 Department of Plant Protection, Faculty of Agriculture, Rasht Branch, Islamic Azad University, Rasht, Iran

2 Department of Plant Protection, Deylaman Institute for High Education, Lahijan, Iran

Abstract

Rice blast disease, which is caused by Pyricularia oryzae, is one of the most important diseases of rice in the world. In this research, out of 150 infected samples collected from rice farms of Guilan province, 20 fungal isolates and 30 bacterial isolates were collected. The isolated bacteria were belonged to Pseudomonas aeruginosa, Enterobacter sp., Enterobacter cloacaeand Bacillus subtilis. Two methods of dual culture and the production of volatile antifungal metabolites were used in the laboratory in order to study biological control. In the greenhouse trial, rice plants (cv Hashemi) were inoculated with the six bacterial isolates as well as P. oryzaepathogen after 12 days. According to the results of this research, in the dual culture method, P. aeruginosa Y–25–3 with 61.11% and in the volatile metabolite method, B. subtilis W with 60.51% were found to be most effective in suppressing mycelial growth of the pathogenic fungus. All isolates significantly alleviated the severity of the P. oryzae–induced disease in greenhouse, but the most effective was P. aeruginosa, which reduced the disease index by 70.53%. Analysis of variance and comparing the characters average in Duncan test and Least Significant Difference (LSD) and based on a completely randomized design revealed significant differences among the bacteria in terms of mycelial inhibition in the dual culture and volatile metabolite methods. Furthermore, in greenhouse trials there were significant differences in the disease index, plant height and fresh weight among the treatments, whereas the treatments did not exhibit any significant differences in dry weight. Based on the results of biocontrol assays in the laboratory and greenhouse, P. aeruginosa Y–25–3 was recognized as the most effective bacterial isolate in the control of rice blast disease.

Keywords

References

Ali, H. & Nadarajah, K. 2014. Evaluating the efficacy of Trichoderma spp. and Bacillus subtilis as biocontrol agents against Magnaporthe grisea in rice. Australian Journal of Crop Science, 8: 1324–1335.
Amini, E., Tajik Ghanbari, M.A. & Hasanzadeh, N. 2012. Biological control of rice blast disease by Streptomyces erythraea invitro and greenhouse conditions. Proceeding of 6th National Conference New Ideas in Agriculture, 29–30 February, Khorasgan, Iran.
Barraquio, W.L., Ladha, J.K. & Watanabe, I. 1983. Isolation and identification of N2–fixing Pseudomonas associated with wetland rice. Canadian Journal of Microbiology, 29(8): 867–873.
Bertrand, P.F. & Gottwald, T.R. 1997. Evaluation of fungicides for pecan disease control. pp. 179–181. In: Hickey, K. D., (ed). Methods for Evaluating Pesticides for Control of Plant Pathogens. Oxford and IHB Publisher, Calcatte, India.
Broadbent, P., Baker, K.F., Franks, N. & Holand, J. 1977. Effect of Bacillus spp. on increased growth of seedlings in steamed and in non–treated soli. Phytopathology, 67: 1027–1034.
Dammie, N. 2017. Biological control of gray leaf spot (Pyricularia grisea (Cooke) Sacc.) of ryegrass. M. Sc. Thesis, University of KwaZulu–Nata. South Africa.
Dhingra, O.D. & Sinclair, J.B. 1995. Basic plant pathology methods. CRC Press, Florida.
Fiddaman, P.J. & Rossal, S. 1993. The production of antifungal volatiles by Bacillus subtilis. Journal of Applied Bacteriology, 74(2): 119–126.
Gnanamanickam, S.S. & Mew, T.W. 1992. Biological control of blast disease of rice (Oryza sativa L.) with antagonistic bacteria and its Mediation by a Pseudomonas antibiotic. Annals of the Phytopathological Society of Japan, 58: 350–358.
Horsfall, J.G. & Barratt, R.W. 1945. An improved grading system for measuring plant diseases. Phytopathology, 35: 655.
IRRI, 1989. Biological control; Blast control. International Rice Research Institute (IRRI), Annual Report for 1988. The Philippines, 231–232.
IRRI. 2013. Standard Evaluation System for Rice. 5th edition. International Rice Research Institute, Manila, Philippines.
Javan Nikkhah, M. 2001. Study on the genetic diversity of Magnaporthe grisea, rice blast pathogen by using molecular, pathogenicity and vegetative compatibility characters in Guilan province. Ph. D. Thesis, University of Tehran. Iran. (In Persian with English summary).
Kazemzadeh Chakoosari, M. 2003. The possibility of biological control of rice sheath blight (Rhizoctonia solani(by some bacterial biocontrol agents. M.Sc. Thesis, Tehran University. Iran. (In Persian with English summary).
Kazemzadeh Chakoosari, M., Rostaei, A., Padasht Dehkaee, F. & Khodakaramian, Q. 2012. Biocontrol of rice sheath blight disease caused by Rhizoctonia solani fungus using certain antagonistic bacteria in the province of Guilan. Journal of Plant Protection, 26: 44–54. (In Persian with English summary).
Khodaparast, S.A. & Sahragard, A. 2004. Rice diseases. The University of Guilan Press. (In Persian with English summary).
Krishnamurthy, K. & Gnanamanickam, S.S. 1998. Biological control of rice blast by Pseudomonas fluorescens strain Pf7–14: Evaluation of a marker gene and formulations. Biological Control, 13(3): 158–165.
Manidipa, R., Gauri Dutta, S. & Venkata, R.C. 2013. Pseudomonads: Potential biocontrol agents of rice diseases. Research Journal of Agriculture and Forestry Sciences, 1(9): 19–25.
Marchesi, J.R., Sato, T., Weightman, A.J., Martin, T.A., Fry, J.C., Hiom, S.J. & Wade, W.G. 1998. Design and evaluation of useful Bacterium–specific PCR primers that amplify genes coding for bacterial 16S rRNA. Applied and Environmental Microbiology, 64: 795–799.
Mu, C., Liu, X., Lu, Q., Jiang, X. & Zhu, C. 2007. Biological control of rice blast by Bacillus subtilis B–332 strain. Acta Phytophylacica Sinica, 34(2): 123–128.
Ningthoujam, D.S., Sanasam, S., Tamreihao, K. & Nimaichand, S. 2009. Antagonistic activities of local actinomycete isolates against rice fungal pathogen. African Journal of Microbiology Research, 3(11):737–742.
Omoboye, O.O., Oni1, F.E., Batool, H., Yimer, H.Z., De Mot, R. & Höfte1, M. 2019. Pseudomonas cyclic Lipopeptides suppress the rice blast fungus Magnaporthe oryzae by induced resistance and direct antagonism. Frontiers in Plant Science, 10: 1–17.
Ou, S.H. 1985. Rice diseases. 2th ed. Commonwealth Mycological Institute.
Padasht Dehkaei, F. & Izadyar, M. 2007. Study on the biological control of rice blast disease in the field conditions. Journal of Agriculture Sciences and Natural Resources, 13: 84–92. (In Persian with English summary).
Padasht Dehkaei, F., Popushoi, I., Izadyar, M., Khodakarmian, G. & Gharyazei, B. 2002. Effects of selected antagonistic bacteria in controlling of rice blast disease. Proceeding of 3th International Rice Blast Conference. 11–14 Sept., Tsukuba, Ibaraki, Japan,
Rosales A.M. & Mew T.W. 1997. Suppression of Fusarium moniliforme in rice by rice–associated antagonistic bacteria. Plant Disease, 81 (1): 49–52.
Rosales, A.M., Mew, T.W. & Nuque, F.L. 1986. Biological control of bakanae disease of rice [Oryza sativa] by antagonistic bacteria [Philippines]. 17th Anniversary and Annual Convention of the Pest Control Council of the Philippines, 8–10 May, Iloilo City, Philippines (abstract).
Rostami, M., Momeni, A., Khosravi, V., Zare, L., Darvishzadeh, N., Omran, M. & Ghalandari, M. 2012. Identification and investigation of effects of antagonistic bacterial agents on rice blast control. Proceeding of 20th Iranian Plant Protection Congress, 25–29 August, Shiraz, Iran.
Safari Motlagh M.R. 2005. Morphological study of Bipolaris species, the causal agent of rice brown spot disease and determination of their genetic variation based on molecular methods, RAPD–PCR and PCR–RFLP in Guilan province. Ph. D. Thesis, Islamic Azad University, Science and Research Branch, Tehran. (In Persian with English summary).
Safari Motlagh, M.R., Padasht Dehkaee, F. & Hedjaroud, G.A. 2005. Rice brown spot disease and evaluation of the response of some rice cultivars to it. Journal of Science and Technology of Agriculture and Natural Resources, 9 (2): 171–182. (In Persian with English summary).
SAS. 2005. SAS/STAT User’s Guide Version 9.1. SAS Institute, Cary, NC, USA.
Sha, Y., Wang, Q. & Li1, Y. 2016. Suppression of Magnaporthe oryzae and interaction between Bacillus subtilis and rice plants in the control of rice blast. SpringerPlus, 5:1238–1251.
Schaad, N.W., Jonse, J.B. & Chun, W. 2001. Laboratory guide for identification of plant pathogenic bacteria.3rd edition, APS Press.
Sherafati, F., Khodaygan, P., Azadvar, M., Sedaghati, E., Saberi–Riseh, R. & Baghaee–Ravari, S. 2014. Association of Pantoea agglomerans with the citrus bacterial canker disease in Iran. Journal of Crop Protection, 3: 345–355.
Sivakumar, D., Wijeratnam, R.W., Wijesundera, R.L.C., Marikar, F.M.T. & Abeyesekere, M. 2000. Antagonistic effect of Trichoderma harzianum on postharvest pathogens of rambutan (Nephelium lappaceum). Phytoparasitica, 28 (3): 240–247.
Spence, C., Alff, E., Johnson, C., Ramos, C., Donofrio, N., Sundaresan, V. & Bais, H. 2014. Natural rice rhizospheric microbes suppress rice blast infections. BMC Plant Biology, 14:130–147.
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. 1997. The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25: 4876–4882.
Wiraswati, S.M., Rusmana, I, Nawangsih, A.A. & Wahyudi, A. T. 2019. Antifungal activities of bacteria producing bioactive compounds isolated from rice phyllosphere against Pyricularia oryzae. Journal of Plant Protection Research, 59(1): 86–94.
Yasmin, S., Hafeez, F.Y., Mirza, M.S., Rasul, M., Arshad, H.M. I., Zubair, M. & Mazhar, I. 2017. Biocontrol of bacterial leaf blight of rice and profiling of secondary metabolites produced by rhizospheric Pseudomonas aeruginosa BRp3. Frontiers in Microbiology, 8: 1–23.

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