Study on morphology and frequency of hemocytes of Blepharopsis mendica (Mantodea: Empusidae)

Document Type : Short Article

Authors

Department of Plant Protection, Faculty of Agriculture, Shahrood University of Technology, Iran

Abstract

Identification of hemocytes is one of the basic studies of circulatory system physiology. Blood cells, as the first defense barrier to insect cellular immunity, participate in blocking the foreign particles in the form of phagocytosis and nodules. In the present study, homocytes of adults of Blepharopsis mendica were identified after staining with Gimsa and using a light microscope with a magnification of 40. In the hemolymph of this predatory insect, five types of homocytes were observed, including prohemocytes, granulocytes, plasmotocytes, oenocytoids, and spherulocytes. The most abundant blood cells in the nymphs and adults of this mantis were spherulocytes and plasmotocytes, respectively. The lowest abundance of cells belonged to prohemocytes. Oenocytoids were the largest cells. Oenocytoids had a large nucleus that extended almost to the side of the cell membrane and the cytoplasmic surface of these cells was granular. Prohemocytes were the smallest cells and observed with the distinct central nucleus. Plasmotocytes were observed as spindles, and the common polymorphic profile of most insects hemolymph was not observed in hemolymph of B. mendica. Granulocytes size were small to medium but were larger than prohemocytes with granule–like granules in cytoplasmic surface. Spherulocytes are medium to large, have a central nucleus, and numerous spherul occupy the cytoplasm of these cells. Adult body weight and blood volume were higher than the nymphs of this mantis. So, it is clear that there are higher populations of hemocytes in adults in comparison nymphs. The identification of mantis homocytes as a large group of pest predators is being investigated for the first time and can be used in the study of its immune features.

Keywords

References

Ajamhassani, M. & Mahmoodzadeh, M. 2020. Cellular defense responses of 5th instar larvae of the Apple Ermine Moth, Yponomeuta malinellus (Lepidoptera: Yponomeutidae) against starvation, thermal stresses and entomopathogenic bacteria Bacillus thuringiensis. Journal of Animal Research, 4(2): 59–68. (In Persian with English summary).
Anonymous, (2014b): http://eol.org/pages/487055/overview (Last update, 09.12.2014).
Baishya, B.P., Bardoloi, S. & Bharali, R. 2015. Ultrastructure of the hemocytes of Muga Silkworm larva Antheraea assama Westwood (Lepidoptera; Saturniidae): a phase–contrast & electron microscopic study. International Journal Pureand Applied Biosceencei, 3(3): 234–240.
Bolu, H. & Ozaslan, C. 2015. Mantis religiosa L. (Mantodae: Mantida). A new host for Podagrion ppachimerum Walker (Hymenoptera: Torymidae) in Turkey. Agriculture & Forestry, 61(2): 183–187.
Correia, A.A. 2008. Histofisiologia do canal alimentar e hemócitos de Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae) tratadas com nim (Azadirachta indica A. Juss). Dissertation, Universidade Federal Rural de Pernambuco.
Ebrahimi, M. & Ajamhassani, M. 2020. Investigating the effect of starvation and various nutritional types on the hemocytic profile and phenoloxidase activity in the Indian meal moth Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae). Invertebrate Survival Journal, 17: 175–185.
Ghasemi, V., Moharramipour, S. & Jalali Sendi, J. 2013. Circulating hemocytes of Mediterranean flour moth, Ephestia kuehniella Zell. (Lep: Pyralidae) and their response to thermal stress. Invertebrate Survival Journal, 10: 128–140.
Giglio, A., Battistella, S., Talarico, F.F., Br&mayr, T.Z. & Giulianini, P.G. 2008. Circulating hemocytes from larvae and adults of Carabus (Chaetocarabus) lefebvrei Dejean 1826 (Coleoptera: Carabidae), Cell types & their role in phagocytosis after in vivo artificial non–self–challenge. Micron, 39(5): 552–558.‏
Gupta, A.P. 1985. Cellular elements in the haemolymph, pp. 85–127. In: Kerkut, G.A. and Gilbert, L.I. (eds.), Comprehensive Insect Physiology, Biochemistry and Pharmacology. Cambridge University Press.
Jones, J.C. 1967. Changes in the hemocyte picture of Galleria mellonella (Linnaeus). The Biological Bulletin, 132(2): 211–221.
Lavine, M.D. & Strand M.R. 2002. Insect hemocytes and their role in immunity. Insect Biochemistry and Molecular Biology, 32: 1295–1309.
Li T, Yan D, Wang X. Zhang L. & Chen P. 2019. Hemocyte changes during immune melanization in Bombyx mori infected with Escherichia coli. Insects, 10(301): 1–15.
Mahmoodzadeh, M., Hakimi tabar, M. & Ajamhassani, M. 2020. Identification of hemocytes and study on hemogram of plum fruit moth Grapholita funebrana (Treitschke) (Lep: Tortricidae). Accepted in Pest Plant Research. (In Persian with English summary).
Mutawa, MY, Ayaad, TH. & SHaurub, EH. 2020. Hemocyte profile, phagocytosis, and antibacterial activity in response to immune challenge of the date fruit stalk borer, Oryctes elegans. Invertebrate Survival Journal, 17: 147–162.
Otte, D.L., Spearman, L. & Stiewe, M.B.D. Mantodea Species file online. 2020. Available online: http://Mantodea.SpeciesFile.org (accessed on 7 January 2020).
Patel, SH., Singh, G. & Singh, R. 2016. Checklist of Global Distribution of Tarachodidae and Toxoderidae (Mantodea: Dictyoptera). International Journal of Contemporary Research and Review, 7(12): 20256–20270.
Pourali, Z. & Ajamhassani, M. 2018. The effect of thermal stresses on the immune system of the potato tuber moth, Phthorimaea operculella (Lepidoptera: Gelechiidae). Journal of Entomological Society of Iran. Supplementary, 37(4): 515–525.
Rabeeie, M.M., Rohani, A, Batiston, R. 2016. Investigation of founestic of Mantodea in south Khorasan province. 22 Iranian Plant Protection Congress. University of Tehran. 459.
 
BioControl in Plant Protection
Volume 8, Issue 1 - Serial Number 15
October 2020
Pages 159-164

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