[Short article] Evaluation of biocontrol potential of Pichia guilliermondii yeast isolated from Urmia Lake in control of postharvest diseases of grapevine

Document Type : Short Article

Authors

1 Plant Protection Research Department, West Azarbaijan Agricultural and Natural Resources Research Center, AREEO, Urmia, Iran

2 Plant Protection Department, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

Abstract

Postharvest diseases cause a lot of damage to grapes and reduce the quality and marketability of the product. These infections are mainly caused by the fungal species Penicillium expansum and Botrytis cinerea, which are the most important pathogen of postharvest grape rot worldwide. Biological control has become important to control these postharvest diseases due to the recent global awareness of the side effects of chemical agents. In this study, the biocontrol ability of yeasts isolated from Urmia Lake basin against postharvest diseases of grapes has been investigated. We used 11 yeasts belonged to Pichia guilliermondii species isolated from Urmia Lake basin. The results of inhibitory activity of 11 P. guilliermondii isolates against two postharvest pathogens of grape, P. expansum and B. cinerea, showed that three yeast isolates can considerably inhibit both fungi in dual culture method on PDA medium. Three promising isolates were selected to reduce postharvest decays of grape at the storage period.  The results exhibited that three yeast isolates could significantly reduce grape decays. The yeast isolate 8 by reducing 82.3% of grape decays, showed the strongest activity against P. expansum. The yeast isolate 10 was found the most effective isolate to suppress B. cinerea decay on grape by 78.5%.

Keywords

References

Antoon, J. & Loomans, M. 2020. Every generalist biological control agent requires a special risk assessment. BioControl, 66: 23–35.
Arras, G. & Arru, S. 1999. Integrated control of postharvest citrus decay and induction of phytoalexins by Debaryomyces hansenii. Adv. Horticulture Science, 13: 76–81.
Barkai–Golan, G. R. 2001. Postharvest disease of fruit and vegetables: Development and control. Amsterdam, NL: Elsevier Science B.V. 418 pp.
Butinar, L., Spencer–Martins, I. & Gunde–Cimerman, N. 2007. Yeasts in high Arctic glaciers: the discovery of a new habitat for eukaryotic microorganisms. Antonie van Leeuwenhoek, 91: 277–289.
Druvefors, U.A. 2004. Yeast biocontrol of grain spoliage moulds–mode of action of Pichia anomala. Doctor٫s dissertation, performed at the Department of Microbiology Swedish University of Agriculture Science. 44pp.
EL–Neshawy, S.M. 1997. Nisin enhancement of biocontrol of postharvest disease of apple with Candida oleophila. Postharvest Biology and Technology, 10: 9–14.
Etebarian, H.R., Sholberg, P.L., Eastwell, K.C. & Sayler, R.J. 2005. Biological control of apple blue mold with Pseudomonas fluorescens. Canadian Journal Microbiology, 51: 591–598.
Freimoser, F.M., Rueda‑Mejia, M.P., Tilocca, B. & Migheli, Q. 2019. Biocontrol yeasts: mechanisms and applications. World Journal of Microbiology and Biotechnology, 35:154.
Janisiewicz, W.J. & Korsten, L. 2002. Biological control of postharvest diseases of fruits. Annual Review of Phytopathology, 40: 411–441.
Jijak, M.H., Lepoivre, P. & Grevesse, C. 1999. Yeast species for biocontrol of apple post–harvest diseases; an encouraging case of study for practical use. In: Upadhyay RK, Mukerji (eds). Biotechnological approaches in biocontrol of plant pathogens. Kluwer Academic/Plenum Publishers, New York, 31–49.
Lillbro, M. 2005. Biocontrol of Penicillium roqueforti on grain–acomparison of mode of action of several yeast species. Master Theisis for the Agriculture Programme, animal science, performed at the Department of Microbiology. Swedish University of Agricultural Sciences, 21pp.
Mokhtarnejad, L., Arzanlou, M. & Babai–Ahari, A. 2015. Molecular and phenotypic characterization of ascomycetous yeasts in hypersaline soils of UrmiaLake basin (NW Iran). Rostaniha, 16: 174–185.
Sharma, R.R., Singh, D. & Singh, R. 2009. Biological control of postharvest diseases of fruits and vegetables bymicrobial antagonists: A review. Biological Control, 50: 205–221.
Zak, J.C. & Wildman, H.G. 2004. Fungi in stressful environments. In: Mueller GM, Bills GF, Foster MS (ed) Biodiversity of fungi, inventory and monitoring methods. Elsevier/Academic, London, 303–315.
Zhimo, V.Y., Biasi, A., Kumar, A., Feygenberg, O., Salim, S., Vero, S., Wisniewski, M. & Droby, S. 2020. Yeasts andBacterial Consortia from Kefir Grains Are Effective Biocontrol Agents of Postharvest Diseases of Fruits. Microorganisms, 8: 428.

Files

Share

How to cite

Statistics