Protective effect of different additives and natural polymers on the stability of Helicoverpa armigera NPV (HaNPV) against ultraviolet radiation

Document Type : Research Paper

Authors

1 Research Center for Modeling and Optimization in Science and Engineering, South Tehran Branch, Islamic Azad University, Tehran, Iran

2 Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

3 Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran

4 Department of Chemical Engineering, South Tehran Branch, Islamic Azad University, Tehran, Iran

5 Agricultural and Natural Resources Research and Education Center, Shahrood, Iran

Abstract

Microbial biopesticides because of their less environmental impact ensure the management of agro–ecosystems. Among the microbial biopesticides, baculoviruses have been interested. Because of the low stability of baculoviruses against sunlight, preparation of UV protectant formulations is being focused in this study. Accordingly, the ultraviolet protective effect of some natural and chemical additives (green tea, black tea, coffee, cocoa, Tween 80, sodium lignin sulfonate and titanium dioxide) at two concentration levels (0.5% and 3% w/v) for the Helicoverpa armigera nucleopolyhedrovirus was evaluated. The investigation was expanded to include three natural polymers (sodium alginate, gelatin, starch) which have recently been used for microencapsulation of biopesticides. Results are based on larval insecticide bioassays to test original activity remaining (OAR). The results showed that titanium dioxide (3% w/v) had no protective effect after 8 hours exposure to UVA (26.66± 3.33). After 48 hours exposure, although titanium dioxide (3% w/v) provided a very little protection against UVA radiation (30.00± 6.35), it still remained the less effective additive. Unlike some reports, titanium dioxide did not provide adequate protection against UVA radiation in our study. In addition, it reduced the activity of occlusion bodies (OBs) in the absence of UV rays. After eight hours exposure to UVA radiation, sodium alginate (3% w/v) was an excellent UVA protectant (93.33± 3.33), whereas the mentioned polymer provided inadequate protection, 48 hours post UVA radiation (50.00± 3.46). The other applied polymers provided medium protection against UVA radiation. After 48 hours, the concentrations of 3% w/v of green tea and coffee provided significant UV protection on stability of Helicoverpa armigera nucleopolyhedrovirus activity at 80.00± 1.54 and 83.33± 3.33, respectively. Lignin at both concentration levels was a proper UV protectant after 48 hours (83.33± 6.66).

Keywords

References

Abbasi, B.H., Khalique, K., Khalique, F., Ayub, N., Liu, H.J., Kazemi, S.A.R. & Naumak, M. 2007. Rearing the cotton bollworm, Helicoverpa armigera on a tapioca based artificial diet. Journal of Insect Science, 7: 35–41.
Arthurs, S.P., Lacey, L.A. & Behle, R.W. 2006. Evaluation of spray–dried lignin based formulations and adjuvants as solar protectants for the granulovirus of the codling moth, Cydia pomonella (L). Journal of Invertebrate Pathology, 93: 88–95.
Arthurs, S.P., Lacey, L.A. & Behle, R.W. 2008. Evaluation of lignins and particle films as solar protectants for the granulovirus of the codling moth, Cydia pomonella. Biocontrol Science and Technology, 18: 829–839.
Behle, R.W. & Popham, H.J.R. 2012. Laboratory and field evaluations of the efficacy of a fast–killing baculovirus isolate from Spodoptera frugiperda. Journal of Invertebrate Pathology, 109: 194–200.
Catena, A.B., Mirón, A.S., Camacho, F.G., Gómez, A.C. & Grima, E.M. 2014. Baculovirus biopesticides: an overview. Journal of Animal and Plant Science, 24: 362–373.
El–Helaly, A., Khattab, M., El–Salamouny, S., El–Sheikh, M. & Elnagar, S. 2013. Promising additives to protect the activity of baculovirus biocontrol agent under field–Sunlight conditions in Egypt. Journal of Life Sciences, 7(5): 495–500.
El–Helaly, A. 2019. Moringa water extract promising additive to prolong the activity of baculovirus under field–sunlight conditions in Egypt. Brazilian Journal of Biology, 1519–6984.
El Salamouny, S., Ranwala, D., Shapiro, M., Shepard, M. & Farrar, R.R. 2009a. Tea, coffee, and cocoa as ultraviolet radiation protectants for the beet armyworm nucleopolyhedrovirus. Journal of Economic Entomology, 102: 1767–1773.
El Salamouny, S., Shapiro, M., Ling, K.S. & Shepard, B.M. 2009b. Black tea and lignin as ultraviolet protectants for the beet armyworm nucleopolyhedrovirus. Journal of Entomological Science, 44: 1–9.
Erayya, Jagdish, J., Sajeesh, P.K. & Upadhyay, V. 2013. Nuclear polyhedrosisvirus (NPV), a potential biopesticide: a review. Research Journal of Agriculture and Forestry Sciences, 1: 30–33.
Farrar, R.R., Shapiro, M. & Javaid, I. 2003. Photostabilized titanium dioxide and a fluorescent brightener as adjuvants for a nucleopolyhedrovirus. BioControl, 48: 543–560.
George, S.E., Ramaiakshmi, K. & Mohan Rao, L.J. 2008. A perception on health benefits of coffee. Critical Reviews in Food Science and Nutrition, 48: 464–486.
Gifani, A., Marzban, R., Safekordi, A.A., Ardjmand, M. & Dezianian, A. 2015. Ultraviolet protection of nucleopolyhedrovirus through microencapsulation with different polymers. Biocontrol Science and Technology, 25(7): 814–827.
Grzywacz, D. & Moore, S. 2017. Production, Formulation, and Bioassay of Baculoviruses for Pest Control. Microbial Control of Insect and Mite Pests, 109–124
Kaiser, D., Bacher, S., Mène–Saffrané, L. & Grabenweger, G. 2019. Efficiency of natural substances to protect Beauveria bassiana conidia from UV radiation. Pest Management Science, 75: 556–563.
Khorramvatan, S., Marzban, R., Ardjmand, M., Safekordi, A.A. & Askary, H. 2013. The effect of polymers on the stability of microencapsulated formulations of Bacillus thuringiensis subsp. kurstaki (Bt–KD2) after exposure to ultra violet radiation. Biocontrol Science and Technology, 24: 462–472.
Kim, H.H., Lee, H., Lee, D., Ko, Y.J., Woo, H., Lee, J., Lee, C. & Pham, A.T. 2020. Activation of Hydrogen Peroxide by a Titanium Oxide–Supported Iron Catalyst: Evidence for Surface Fe (IV) and Its Selectivity. Environmental Science and Technology, 54(23): 15424–15432.
Kumar, J., Ramlal, A., Mallick, D. & Mishra, V. 2021. An Overview of some biopesticides and their importance in plant protection for commercial acceptance. Plants, 10: 1185.
Leonardo, T.S., Rodrigo, T., Leonardo, P.C., Marcos, F. & Gilberto, M.A.F. 2007. The influence of brown algae alginates on phenolic compounds capability of ultraviolet radiation absorption in vitro. Brazilian Journal of Oceanography, 55 (2): 145–154.
Pellegrini, N., Serafini, M., Colombi, B., Del Rio, D.D., Salvatore, S., Bianchi, M. & Brighenti, F. 2003. Total antioxidant capacity of plant foods, beverages and oils consumedin Italy assessed by three different in vitro assays. Journal of Nutrition, 133: 2812–2819.
Ruiu, L. 2018. Microbial biopesticides in agroecosystems. Agronomy, 8: 235.
Satinder, K.B., Verma, M., Tyagi, R.D. & Vale´ro, J.R. 2006. Recent advances in downstream processing and formulations of Bacillus thuringiensis based biopesticides. Process Biochemistry, 41: 323–342.
Sayed, W.A.A., El–Bendary, H. & El–Helaly, A. 2020. Increasing the efficacy of the cotton leaf worm Spodoptera littoralis nucleopolyhedrosis virus using certain essential oils. Egyptian Journal of Biological Pest Control, 30(8).
Shapiro, M., El Salamouny, S. & Shepard, B.M. 2008. Green tea extracts as ultraviolet protectants for the beet armyworm, Spodoptera exigua, nucleopolyhedrovirus. Biocontrol Science and Technnology, 18: 591–603.
Shapiro, M., El Salamouny, S. & Shepard, B.M. 2009. Plant extracts as ultraviolet radiation protectants for the Beet Armyworm (Lepidoptera: Noctuidae) nucleopolyhedrovirus: screening of extracts. Journal of Agricultural and Urban Entomology, 26: 47–61.
Shapiro, M., El Salamouny, S., Shepard, B.M. & Jackson, D.M. 2009. Plant phenolics as radiation protectants for the Beet Armyworm (Lepidoptera: Noctuidae) nucleopolyhedrovirus. Journal of Agricultural and Urban Entomology, 26: 1–10
Shapiro, M., Shepard, B.M. & Lopez, R. 2007a. Effect of spices upon the activity of the gypsy moth (Lepidoptera: Lymantriidae) nucleopolyhedrovirus. Journal of Entomological Science, 42: 84–91.
Shapiro, M., Shepard, B.M. & Lopez, R. 2007b. Effects of medicinal herbs on the biological activity of the gypsy moth nucleopolyhedrovirus. Journal of Entomological Science, 42: 426–429.
Shasha, B.S., McGuire, M.R. & Behle, R.W. 1998. Lignin based pest control fonnulations. U.S. Patent No. 5,750,467.
Shin, S., Wang, L.X., Zheng, X.Q., Xiang, L.P. & Liang, Y.R. 2014. Protective effect of epigallocatechin gallate against photo–damage induced by ultraviolet an in human skin fibroblasts. Tropical Journal of Pharmaceutical Research, 13 (7): 1079–1084.
Sutanto, K.D., ElSalamouny, S., Tufail, M., Rasool, K.G., Sukirno, S., Shepard, M., Shapiro, M. & Aldawood, A.S. 2017. Evaluation of natural additives to enhance the persistence of Spodoptera littoralis (Lepidoptera: Noctuidae) Nucleopolyhedrovirus (SpliMNPV) under field conditions in Saudi Arabia. Journal of Economic Entomology, 1–7.
Tamez–Guerra, P., Michael, R.M., Behle, R.W., Hamm, J.J., Sumner, H.R. & Shasha, B.S. 2000. Sunlight persistence and rain fastness of spray–dried formulations of baculovirus isolated from Anagrapha falcifera (Lepidoptera: Noctuidae). Journal of Economic Entomology, 93: 210–218.
Tufts, D.M., Spencer, K., Hunter, W.B. & Bextine, B. 2011. Delivery system using sodium alginate virus loaded pellets to red imported fire ants (Hymenoptera: Formicidae). Florida Entomologist, 94: 237–241.
Villamizar, L., Barrera, G., Cotes, A.M. & Martinez, F. 2010. Eudragit S100 microparticles containing Spodopterafrugiperdanucleopolyehedrovirus: Physicochemical characterization, photostability and in vitro virus release. Journal of Microencapsulation, 27: 314–324.
Vishnoi, H., Bodla, R.B. & Kant, R. 2018. Green tea (Camellia sinensis) and its antioxidant property: a review. International Journal of Pharmaceutical Sciences and Research, 9(5): 1723–36.
Wilson, K., Grzywacz, D., Curcic, I., Scoates, F., Harper, K., Rice, A., Paul, N. & Dillon, A. 2020. A novel formulation technology for baculoviruses protects biopesticide from degradation by ultraviolet radiation. Scientific Reports, 10: 13301.

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