Study of the inhibitory effects of hydro–alcoholic extract and nanoemulsion essential oil of Khuzistani savory on local lesion induction by cucumber mosaic virus (CMV) on cowpea

Document Type : Research Paper

Authors

1 Department of Plant Protection, College of Agriculture Sciences and Food Industries, Science and Research Branch, Islamic Azad university, Tehran, Iran

2 Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C. Evin, Tehran, Iran

3 Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C. Evin, Tehran, Iran

Abstract

Plant viruses are a limiting factor in agriculture. Natural compounds, especially herbal plant metabolites, have been considered to control viruses. In this regard, the effect of aqueous–ethanolic extract (50% water) and essential oil nanoemulsion of Satureja khuzistanica were investigated to reduce the infection of cucumber mosaic virus on the local lesion test plant of cowpea (Vigna unguiculata) in greenhouse conditions. The extract and essential oil nanoemulsion were applied in four concentrations of 0.5, 1, 2 and 4 percent (g/100 mL) and three different times including simultaneously, two hours before, and after inoculation of plant leaves with virus inoculum (concentration 2 mg/ml). The lowest number of local lesions was obtained by using 2 and 4% of hydro–ethanolic extract (by 66–78% disease reduction) and nanoemulsion of essential oil with a concentration of 2% (by 59% disease reduction), and two hours after inoculation with virus inoculum. The particle size of the nanoemulsion after preparation was 119.4 nm, which reached 232.8 nm after 60 days of storage, which indicates the good physical stability of the nanoemulsion. According to GC–MS analysis, the main constituents of essential oil were carvacrol (91.5%), para–cymene (1.5%), beta–caryophyllene (1.1%) and beta–pinene (1.1%). Nanoemulsion of this essential oil improved its efficiency to inhibit the cucumber mosaic virus on cowpea. This study is the first report of the antiviral effect of phytochemical compounds of Khuzistani savory to inhibit a plant virus.

Keywords

References

Abbaszadeh, M. 2005. Determining the effect of weight loss diet alone and with Satorex on weight control. Thesis of General Medicine, Lorestan University of Medical Sciences.
Adam, R. 2007. Identification of essential oil components by gas chromatography/mass spectrometry. Illinois: Allure Publishing Corporation.
Agrios, G.N. 2005. Plant Pathology. 5th ed. Elsevier Academic Press, London. 
Ahmadi, Sh., Sefikan, F., Babakhanlo, P., Asgari, F., Khademi, K., Valizadeh, N. & Karimifar, M.A. 2009. Comparison of compounds in (Satureja bachtiarica Bunge) essential oil in the pre–flowering and full flowering stages in the habitat and field. Iranian Journal of Medicinal and Aromatic Plants Research, 25: 169–159.
Alishiri, A. & Rakhshandehroo, F. 2015. Study on the antiviral activity of aqueous pokeweed (Phytolacca americana L.) extracts against Potato virus Y. Iranian Journal of Medicinal and Aromatic Plants, 31: 479–488.
Dadkhah, F. 2003. Anti–inflammatory and analgesic effects of Khuzestani savory. PhD Thesis in General Pharmacy, Tehran University of Medical Sciences.
Delmas, T., Piraux, H., Couffin, A.C., Vinet, F., Poulin, P., Cates, M.E. & Bibette, J. 2011. How to prepare and stabilize very small nanoemulsions. Langmuir : the ACS Journal of Surfaces and Colloids, 27: 1683–1692.
Dosti, B. 2005. Investigation of structure, metastructure and tuberous growth of Khuzestani safflower and analysis of essential oil compounds during vegetative and reproductive stages of the plant and its antimicrobial properties. PhD Thesis in Biology, Tehran Research Sciences Branch, Islamic Azad University of Tehran.
Dunkie, V., Bazic, N., Vuko, E. & Cukrov, D. 2010. Antiphytoviral Activity of Satureja montana L.ssp.variegata (Host)P.W. Ball Essentional Oil and Phenol Compounds on CMV and TMV. Molecules, 15: 6713–6721.
Farsam, H., Manlou, M., Radpour, M.R., Salehinia, A.N. & Shafiee, A. 2004. Composition of the essential oils of wild and cultivated Satureja khuzistanica Jamzad from Iran. Flavour and Fragrance Journal, 19: 308– 310.
Ghasemi, S. & Tabein, S. 2013. Evaluation of antiviral effects of plant extracts against Tobacco mosaic virus. Plant Protection Journal (Islamic Azad University, Shiraz Branch), 6: 353–363.
Ghosh, V., Mukherjee, A. & Chandrasekaran, N. 2013. Ultrasonic emulsification of food–grade nanoemulsion formulation and evaluation of its bactericidal activity. Ultrasonics Sonochemistry, 20: 338–344.
Hadian, J., Mirjalili, M.H., Kanani, M.R., Salehnia, A. & Ganjipoor, P. 2011. Phytochemical and morphological characterization of Satureja khuzistanica Jamzad populations from Iran. Chemistry & Biodiversity, 8: 1–15.
Hyldgaard, M., Mygind, T. & Meyer, R.L. 2012. Essential oils in food preservation: mode of action, synergies, and interactions with food matrix components. Frontiers in Microbiology, 3(25): 12.
Kemertelidze, É.P., Sagareishvili, T.G., Syrov, V.N. & Khushbaktova, Z.A. 2004. Chemical composition and pharmacological activity of garden savory (Satureja hortensis L.) occuring in Georgia. Pharmaceutical Chemistry Journal, 38: 319–322.
Kirimer, N., Baer, K.H.C. & Tiimen, G. 1995. Carvacrol rich plant in Turkey. Chemistry of Natural Compounds, 31: 37–41.
Manoharan, M.J., Bradeeba, K., Rengasamy, P., Palanivel, K.S., Puneet, S.C., Sherlyn, T., Abitha, B. & Tongmin, S. 2012. Development of surfactin based nanoemulsion formulation from selected cooking oils: Evaluation for antimicrobial activity against selected food associated microorganisms. Journal of the Taiwan Institute of Chemical Engineers, 43: 172–180.
Moghimi, R., Ghaderi, L., Rafati, H., Aliahmadi, A. & McClements, D.J. 2016. Superior antibacterial activity of nanoemulsion of Thymus daenensis essential oil against E. coli. Journal of Agricultural and Food Chemistry, 194: 410–415.
Omid baigi, R. 2005. Production and Processing of Medicinal Plants.  3rd edition, with complete revision) Astan Quds Razavi Publication, Tehran.
Othman, B.A. & Shoman, S.A. 2004. Antiphytoviral Avtivity of the Plectranthus tenuiflorus on Some Important Viruses. International Journal of Agricuiture & Biology, 6: 844–849.
Petersen, M. & Simmonds, M.S.J. 2003. Molecules of interest: Rosmarinic acid. Phytochemistry, 62: 121–125.
Pourhosein alamdari, M., 1391. Nanoemulsion formulation of Satureja khuzistanica essential oil with the aim of treating sinusitis and phytochemical study of Echinacea purpurea and its protective effect on PC12 cells. Master Thesis, Research Institute of Medicinal Plants and Raw Materials, Shahid Beheshti University.
Rakhshandehroo, F., Modarresi, A. & Zamani Zadeh, H.R. 2009. Study on the antiviral effect of aquatic and alcoholic extracts of Urtica dioica L. on rose mosaic viral disease in vitro culture. Iranian Journal of Medicinal and Aromatic Plants, 25: 403–413.
Rasoulpour, R., Afsharifar, A. & Izadpanah, K. 2018. Antiviral activity of prickly pear (Opuntiaficus–indica (L.) Miller) extract; Opuntin B, a second antiviral protein. Crop protection, 112: 1–9.
Salahvarzi, S. 2005. Investigation of chemical compounds in Khuzestani savory extract. Master Thesis, Sharif University of Technology, Tehran.
Sefikan, F., Sadeghzadeh, L., Teymouri, M., Asgari, F. & Ahmadi, Sh. 2007. Investigation of antimicrobial effects of essential oils of two savory species (Satureja khuzestanica Jamzad and Satureja bachtiarica Bunge) in two stages of harvest. Iranian Journal of Medicinal and Aromatic Plants Research, 23: 182–174.
Sugumar, S., Nirmala, J., Ghosh, V., Anjali, H., Mukherjee, A. & Chandrasekaran, N. 2013. Bio–based nanoemulsion formulation, characterization and antibacterial activity against food–borne pathogens. Journal of Basic Microbiology, 53: 677–685.
Wagner, H., Wierer, M. & Bauer, R. 1986. In vitro inhibition of prostaglandin biosynthesis by essential oils and phenolic compounds. Planta Medica, 3: 184–187.
Zittre, T.A. & Gonsalves, D. 1991. Differentiation of pseudorecombinants of two cucumber mosaic virus starins by biological properties and aphid transmission. Phytopathology, 81: 139–143.
Verma, H.N., Srivastava, S. & Kumar, D.1996.Induction of systemic resistance in plant against viruses by a basic protein from Clerodendrum aculeatum leaves. Phytopathology, 85:485–492.

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