Identification and characterization of endophytic bacterium, Pseudomonas fluorescens NZ105 as biocontrol agent of Verticillum wilt of tomato

Document Type : Research Paper

Authors

1 Department of Plant Protection, Faculty of Agricultural Sciences and Food Industries, Science & Research Branch, Islamic Azad University, Tehran, Iran

2 Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

Abstract

Tomato (Lycopersicon esculentum) is one of the most important crops in the world and Iran. Verticillium wilt of tomato caused by V. dahliae is one of the most important obstacles to the cultivation of this plant in the country. Using endophytes has been introduced as a new solution to plant disease management. The aim of this study was to introduce endophytic bacteria as biological control agent for Verticillium wilt of tomato. For this purpose, plants were collected from both greenhouses and tomato fields in Tehran and Alborz provinces. Endophytic bacteria were isolated mainly from the roots, stems, leaves and fruits of tomato. Based on key tests and fluorescent pigmentation on King' B medium, 30 fluorescent Pseudomonas were selected. In dual culture tests, six isolates with biocontrol capacity of V. dahliae were screened and complementary evaluation was done under greenhouse conditions. The P. fluorescens NZ105 strain was identified as the most effective biocontrol agent with 3.7 mm inhibition zones in Petri dish and 85% disease incidence reduction in greenhouse. This strain was identified based on the key tests and 16S rDNA gene amplification. The results of the greenhouse test showed that P. fluorescens NZ105 had the best performance in increasing plant growth factors including plant dry weight, dry root weight, plant height, root length, wet root weight and wet plant weight. Furthermore, the studies showed that this strain is capable of producing protease, volatile antifungal metabolites and extracellular metabolites.

Keywords

References

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