Changes in hemocytes of Plutella xylostella (Lepidoptera:Plutellidae) in response to Bacillus thuringiensis and thermal stresses

Document Type : Research Paper

Authors

Department of Plant Protection, Faculty of Agriculture, Shahrood University of Technology, Iran

Abstract

The circulatory system is involved in the transport and storage of food and defense against microorganisms. The success rate of this system depends on the number and type of blood cells and their proper function against contaminants in the body. In the present study, the effect of Bacillus thuringiensis and temperature stress was investigated on homocyte abundance and phenol oxidase activity in fifth larvae of Plutella xylostella. In the first experiment, rapeseed leaves were treated with sub–lethal concentrations of bacteria and fifth instar larvae were bled at 12 and 24 hours after feeding on the leaves. In the second experiment, the larvae were exposed to 4 and 30 degrees Celsius for 12 hours and temperature stress was examined on their homocytes. The results showed that feeding on canola leaves infected with bacteria after 12 and 24 hours caused a significant increase in total cells, plasmotocytes and granulocytes and phenol oxidase activity compared to the control. Temperature stresses also increased the number of homocytes and phenol oxidase activity compared to the control, which was more significant in larvae that tolerated high temperature stress. Certainly, additional research is needed to be able to evaluate the possible use of microbial control methods against cabbage willow by recognizing the safety characteristics.

Keywords

References

Ajamhassani, M., Sendi, J.J., Zibaee, A., Askary, H. & Farsi, M.J. 2013. Immunoliogical Responses of Hyphantria Cunea (Drury) (Lepidoptera: Arctiidae) to Entomopathogenic Fungi, Beauveria Bassiana (Bals.–Criy) and Isaria Farinosae (Holmsk.) Fr. Journal of Plant Protection Research, 53: 110–118.
Ajamhassani, M. 2014. Study on cellular defense of larvae of Utethesia pulchella (Lepidoptera: Arctiidae) against to Beauveria bassiana and Isaria farinosae. Biocontrol in Plant Protection, 2(1): 57–67. (In Persian with English summary).
Ajamhassani, M. 2015. Study of cytology of hemocytes in the Spurge hawk–moth, Hyles euphorbiae L. (Lepidoptera: Sphingidae). Plant Protection (Agricultural Science Journal), 38(3): 49–62. (In Persian with English summary)
Ajamhassani, M. 2019. Study on morphology and frequency of hemocytes in Osphranteria coerulescense (Redt) (Coleoptera: Cerambycidae) and Zeuzera pyrina L. (Lepidoptera: Cossidae) larvae, two wood boring insects of Iran. Iranian Journal of Forest and Range Protection Research, 17(2): 96–106. (In Persian with English summary).
Ajamhassani, M. & Pourali, Z. 2020. Hemogram study and effect of thermal Stresses on abundance of immunocytes in larvae of Goat Moth, Cossus cossus (Lepidoptera: Cossidae). Iranian Journal of Forest and Range Protection Research, 17(2): 239–250. (In Persian with English summary).
Ajamhassani, M. & Mahmoodzadeh, M. 2020. Cellular defense responses of 5th instar larvae of the Apple Ermine Moth, Yponomeuta malinellus (Lepidoptera: Yponomeutidae) against starvation, thermal stresses and entomopathogenic bacteria Bacillus thuringiensis. Journal of Animal Researches, 4(2): 59–68. (In Persian with English summary).
Ajamhassani, M. 2021. Hemocyte changes of larvae of the beet moth, Scrobipalpa ocellatella (Lepidoptera: Gelechiidae) affected by thermal stress. Journal of Entomological Society of Iran, 41(1):101–103. (In Persian with English summary).
Bao, Y., Yamano, Y. & Morishima, I. 2007. Induction of hemolin gene expression by bacterial cell wall components in eri–silkworm, Samia cynthiaricini. Molecular Biology, 146: 147−151.
Beckage, N. E. 2008 Insect Immunology, Academic Press. California.
Blanco, L.A.A., Crispim, J.S., Fernandes, K.M., de Oliveira, L.L., Pereira, M.F., Bazzolli, D.M.S. & Martins, G.F. 2017. Differential cellular immune response of Galleria mellonella to Actinobacillus pleuropneumoniae. Cell and Tissue Research, 370(1): 153–168.
Borges, A.R., Santos, P.N. Furtado, A.F. & Figueiredo, R.C. 2008. Phagocytosis of latex beads and bacteria by hemocytes of the triatomine bug Rhodnius prolixus (Hemiptera: Reduvidae). Micron, 39: 486–49.
Correia, A. A., Wanderley–Teixeira, V., Oliveira, J.V. & Torres, J.B. 2008. Dinámica hemocitaria en larvas de Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) tratadas con nim (Azadirachta indica A. Juss). Boletín de Sanidad Vegetal Plagas, 34: 357–365.
Duarte, J.P., Silva, C.E., Ribeiro, P.B. & Carcamo, M.C. 2020. Do dietary stresses affect the immune system of Periplaneta americana (Blattaria: Blattidae)? Brazillian Journal of Biology, 80(1): 73–80.
Ebrahimi, M. & Ajamhassani, M. 2020. The Effects of Starvation Challenges and Nutritional Diets on Immunity System of Indian Meal Moth Plodia interpunctella (Hubner) (Lepidoptera Pyralidae). Invertebrate Survival Journal, 17: 175–185.
Fahimi, A., Kharrizi–pakdel, A. & Talaee–Hassanloui, R. 2008. Evaluation of effect of PxGV–Taiwanii on cabbage moth Plutella xylostella (Lepidoptera: Plutellidae) in laboratory conditions. Pakistan Journal of Biological Sciences, 11(13): 1768– 1770.
Ferré, J. & Van Rie, J. 2002. Biochemistry and genetics of insect resistance to Bacillus thuringiensis. Annual Review of Entomology, 47: 501–533.
Ghasemi, V., Moharramipour, S. & Jalali Sendi, J. 2013. Circulating hemocytes of Mediterranean flour moth, Ephestia kuehniella Zell. (Lep: Pyralidae) and their response to thermal stress. Invertebrate Survival Journal, 10: 128–140.
Gillespie, J.P., Burnett, C. & Charnley, A.K. 2000. The immune response of the desert locust Schistocerca gregaria during mycosis of the entomopathogenic fungus, Metarhizium anisopliae var acridum. Journal of Insect Physiology, 46: 429–437.
Gupta, A.P. 1985. Cellular elements in the hemolymph. Comprehensive Insect Physiology Biochemistry and Pharmacology, 3: 401–451.
Hannon, E.R., Rodstrom, R.A., Chong, J.M. & Brown, J.J. 2017. Carpenterworm Moth. Washington State University Extension.
Hernandez, S., Lanz, H., Rodriguez, M.H., Torres, J.A., Martinez, P.A. & Tsutsumi, V. 1999. Morphological and cytochemical characterization of female Anopheles albimanus (Diptera: Culicidae) hemocytes. Journal of Medical Entomology, 36: 426–434.
Huang, F., Shi, M. & Yang, Y. 2009. Changes in hemocytes of Plutella xylostella after parasitism by Diadegma semiclausum. Archives of Insect Biochemistry and Physiology, 70(3): 177–187.
Jiang, H., Wang, Y., Ma, C. & Kanost, M.R. 1997. Subunit composition of pro–phenol oxidase from Manduca sexta: molecular cloning of subunit ProPO–P1. Insect biochemistry and molecular biology, 27(10): 835–850.
Jones, J.C. 1967. Changes in the hemocyte picture of Galleria mellonella L. Biological Bulletin, 132: 211–221.
Lavin, e, M.D. & Strand, M.R. 2002. Insect hemocytes and their role in immunity. Insect Biochemistry and Molecular Biology, 32(10): 1295–1309.
Leonard, C., Soderhall, K.N. & Ratcliffe, A. 1985. Studies on prophenoloxidase and protease activity of Balbifer cranifer haemocytes. Insect Biochemistry, 15: 803–810.
Li, T., Yan, D., Wang, X., Zhang, L. & Chen, P. 2019. Hemocyte Changes During Immune Melanization in Bombyx Mori Infected with Escherichia coli. Insects, 10(301): 1–15.
Lubawy, J. & Slocinska, Malgorzata. 2020. Characterization of Gromphadorhina coquereliana hemolymph under cold stress. Scientific Reports.
Ma, G., Roberts, H., Sarjan, M., Feath erstone, N., Lahnstein, J., Akhurst, R. & Schmidt, O. 2005. Is the mature endotoxin Cry1Ac from Bacillus thuringiensis inactivated by a coagulation reaction in the gut lumen of resistant Helicoverpa armigera larvae? Insect Biochemistry and Molecular Biology, 35 (7): 729–739.
Manjula, P., Lalitha, K. & Shivakumar, M.S. 2020. Diet composition has a differential effect on immune tolerance in insect larvae exposed to Mesorhabditis belari, Enterobacter hormaechei and its metabolites. Journal of Experimental Parasitology, 208: 1–7.
Mowlds, P. & Kavanagh, K. 2008. Effect of pre–incubation temperature on susceptibility of Galleriamellonella larvae to infection by Candida albicans. Mycopathologia, 165: 5–12.
Nakahara, Y., Kanamori, Y., Kiuchi, M. & Kamimura, M. 2003. In vitro studies of hematopoiesis in the silkworm: Cell proliferation in and hemocyte discharge from the hematopoietic organ. Journal of Insect Physiology, 49: 907–916.
Negreiro, M.C.C., Andrade, F.G.D. & Falleiros, Â.M.F. 2004. Sistema imunológico de defesa em insetos: uma abordagem em lagartas da soja, Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae), resistentes ao AgMNPV. Semina: Ciências Agrárias, 25(4): 293–308.
Söderhäll, K. & Cerenius, L. 1998. Role of the prophenoloxidase–activating system in invertebrate immunity. Current Opinion in Immunology, 10(1): 23–28.
Pourali, Z. & Ajamhassani, M. 2018. The effect of thermal stresses on the immune system of the potato tuber moth, Phthorimaea operculella (Lepidoptera: Gelechiidae). Journal of Entomological Society of Iran.Supplementary, 37(4): 515–525.
Rahman, M.M., Roberts, H.L.S., Sarjan, M., Asgari, S. & Schmidt, O. 2004. Induction and transmission of Bacillus thuringiensis tolerance in the flour moth Ephestia kuehniella. . Proceedings of the National Academy of Sciences of the United States of America, 101(9): 2696–2699.
Ribeiro, L., Teixeira, V., Cunha, F., Teixeira, A. & Siqueira, H. 2012. Immunological response of resistant and susceptible Plutella xylostella (Lepidoptera: Plutellidae) to Bacillus thuringiensis. Revista Colombiana de Entomología, 38(2): 208–214.
Sayyed, A.H., Raym ond, B., Ibiza–Palacios, M.S., Escriche, B. & Wright, D.J. 2004. Genetic and biochemical characterization of field–evolved resistance to Bacillus thuringiensis toxin Cry1Ac in the diamondback moth, Plutella xylostella. Applied and Environmental Microbiology, 70(12): 7010–7017.
Shamakhi, L., Zibaee, A., Karimi, A. & Hoda, H. 2019. Effect of thermal stress on the immune responses of Chilo suppressalis walker (Lepidoptera: Crambidae) to Beauveria bassiana. Journal of Thermal Biology, 84: 136–145.
Stanley, D. & Miller, J.S. 2006. Eicosanoid actions in insect cellular immune functions. Entomologia Experimentalis ET Applicata, 119: 1–13.
Strand, M.R. 2008. Insect hemocytes and their role in immunity. Insect immunology, 25–47.
Vengateswari, G., Arunthirumeni, M. & Shivakumar, M.S. 2020. Effect of food plants on Spodoptera litura (Lepidoptera: Noctuidae) larvae immune and antioxidant properties in response to Bacillus thuringiensis infection. Toxicology Reports, 1428– 1437.
Whalon, M.E., Mota –Sanchez, D. & Hollingworth, R.M. 2008. Analysis of global pesticide resistance in arthropods. p. 5–31. In: Whalon, M. E. (Ed.). Global Pesticide Resistance in Arthropods. CABI Publishing. Wallingford. United States of America. 192 p.
Zhao, J.Z., Collins, H.L., Li, Y.X., Mau, R.F.L., Thompson, G.D., Hertlein, M. Andaralo, J.T., Boykin, R. & Shelton, A.M. 2006. Monitoring of diamondback moth (Lepidoptera: Plutellidae) resistance of spinosad, indoxacarb, and emamectin benzoate. Journal of Economic Entomology, 99(1): 176–181.

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