Fermentation and formulation of Trichoderma asperellum for biological control of sugar beet damping–off disease

Document Type : Research Paper

Authors

Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension (AREEO), Tehran, Iran.

10.22092/bcpp.2022.128528

Abstract

Biological control of plant diseases using bacterial and fungal antagonists has been accepted as a suitable alternative for the chemical control which is harmful for human health and it is also a contaminant of the agricultural environment. One of the important limitations of biocontrol agents is the ineffectiveness in the field conditions which is related to the lack of stability and duration of biocontrol agents that can mostly be due to the lack of proper formulations.  In this research study, four new bioformulations were developed and prepared using two strains of a fungal antagonist (Trichoderma asperellum), rice bran as the substrate, Kaolin as the carrier and carboxy methyl cellulose and arabic gum as the stickers.  For the development of bioformulations, rice bran substrate was mixed with spore suspension of two most effective strains of T. asperellum . Plastic bags containing rice bran and fungal antagonists spore suspension were incubated for two months at 250C until the fungus covered the whole surface of the substrate in the bags (Fermentation phase). The containing of the bags was then evacuated and the powdery colonized substrate was used for the development of final bioformulations. The bioformulations were developed and produced by mixing Kaolin powder as the carrier by 20% of the substrate weight and two stickers including Carboxy Methyl Cellulose (CMC) and Arabic Gum (AG) each by 1% of substrate weight.  Four bioformulations were produced with the identification codes of Ka–CMC–Ta–4, Ka–AG–Ta–4, Ka–CMC–Ta–5 and Ka–AG–Ta–5 respectively.  In addition, the shelf life and some important physical and chemical properties of the bioformulations including percent humidity, pH and their suspensibility were also determined.

Keywords

References

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