Investigating the efficiency of four strains of Bacillus spp. in biological control of strawberry anthracnose disease

Document Type : Research Paper

Authors

Department of Plant Protection, College of Agriculture, University of Kurdistan, Sanandaj, Iran

10.22092/bcpp.2022.128711

Abstract

Endophytic bacterial strains, isolated from healthy strawberry seedlings (Fragaria × ananassa) collected in Kurdistan province and its antifungal activity against Colletotrichum nymphaeae, causal agent strawberry anthracnose was evaluated under in vitro, in vivo, and greenhouse conditions. Bacterial strains were identified as Bacillus spp. using biochemical properties and molecular phylogenetic analysis of the 16S rDNA gene sequences. The results of biocontrol tests in vitro showed that the bacterial strains had an effective antifungal activity against C. nymphaeae of strawberry anthracnose. All strains represented in vitro antifungal activity against C. nymphaeae by dual culture test with (64.04%) in MarG19, (60.59%) in SN3, (61.57%) in DM6 and (54.18%) LN1. As well as, the cell–free–culture filtrates of bacterial strains decreased the mycelial growth and conidial germination of pathogen and inhibition was assayed in MarG19 (79.02%), in SN3 (41.95%), in DM6 (32.86%) and in LN11 (34.96%) on mycelial growth and inhibitory percentage with 86.31% in MarG19, 75.33% in SN3, 41.10% in DM6 and 76.73% in LN11 on conidial germination. Also, volatile compounds (VOCs) partially reduced the mycelium growth of C. nymphaeae. The in vivo test on fruit was done and decreased disease severity by 71/19% in MarG19, 76/15% in SN3, 88.07% in DM6 and 85.43% in LN11. The biocontrol efficacy under greenhouse conditions was measured using drenching soil and inoculated plants. The highest inhibitory percentage in DM6 and MarG19 was (77.77%) in drenching soil method and in SN3 was (100%) in plant inoculation method.

Keywords

References

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