Optimizing the production of biological pesticide Bacillus thuringiensis by Taguchi method

Document Type : Research Paper

Authors

1 -

2 Member of Scientific Board, Iranian Research Institute of Plant Protection

3 Department of Agricultural Entomology, Islamic Azad University, Science and Research Branch, Tehran, Iran.

4 Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization

10.22092/bcpp.2023.362185.332

Abstract

After obtaining the superior Bt strains in terms of pathogenicity and host range, it is of particular importance to provide low-cost culture media for the production of spores and crystals in laboratory and mass production in industrial-scale bioreactors. In this research, an attempt has been made to provide a suitable and optimal food substrate for the growth of Bt by using cheap inputs such as waste and by-products of agricultural industries, and to evaluate the production rate of this bacterium using these materials. In this research, the waste and by-products of agricultural industries were investigated as culture medium in the form of extract and in liquid form for the propagation of native Bt strain (6R). The culture media were evaluated as a combination of two and three media with different percentages and the optimization process was done with Taguchi method and using Qualitek-4 software. The results of the experiments and data analysis showed that in the culture medium with two substance, beet molasses combined with corn steep with 4.22 × 1010 CFU/ml and wheat bran combined with corn steep with 3.44 × 1010 CFU/ml, and the culture medium with tree substance including soy, corn steep, fish meal 3.98 × 1016 CFU/ml, soy, corn steep, sugar cane molasses 9.89 × 1016 CFU/ml and wheat bran, corn steep, sugar beet molasses 9.29 × 1016 CFU/ml were the best culture media that had the highest production of Bt in laboratory conditions. The results showed that Bt obtained from culture medium with two substance of sugar beet molasses and corn steep, wheat bran and corn steep and culture medium with tree substance of soybean, corn steep and fish meal; corn steep, soybean and sugarcane molasses; corn steep, wheat bran and sugar beet molasses caused 100% mortality on the 3 instar larvae of Spodoptera littoralis

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References

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BioControl in Plant Protection
Volume 10, Issue 1
August 2022
Pages 143-153

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