Investigation of mortality rate of alfalfa stem nematode Ditylenchus dipsaci under the influence of saponins of some alfalfa ecotypes (Medicago sativa)

Document Type : Research Paper

Authors

1 Associated Professor (Retired), Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

2 Former Ph.D. Graduated, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.

3 Assistant Professor, Department of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

4 Associated Professor,Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.

10.22092/bcpp.2023.363525.346

Abstract

Alfalfa stem nematode (Ditylenchus dipsaci) is one of the most important and destructive pests of economic crops such as alfalfa, garlic, onion and tulip. The effects of Saponins from the aerial parts of six alfalfa ecotypes on the mortality of alfalfa stem nematode was investigated under laboratory conditions. In this study. The results showed that among the crude Saponin treatments, the amount of 90 microliters from the Hamedani and Faiz ecotypes had the highest effect with 96% and the amount of 50 microliters from the local Miandoab ecotype 42% had the least effect on the nematode mortality. Also, among the pure Saponins, the amount of 50 microliters of Hamedani, Shiraz–Plycross and Faiz ecotypes with 99%, and the amount of 10 microliters of Nishaburi ecotype with 68% had the highest and lowest effect, respectively, on the mortality of the nematode. In most ecotypes, increasing the concentrations of crude Saponin from 50 to 90 microliters and pure Saponin from 10 to 50 microliters causing 54% and 31% mortalities led to an increase in nematode mortality. It means there is a positive correlation between alfalfa stem nematode mortality and the raw and pure Saponin concentration of alfalfa ecotypes. Subsequently, Hamedani, Faiz and Polycross Shiraz ecotypes showed the highest effect and Miandoab and Nishaburi local ecotypes showed the least effect on the mortality of this nematode.
 
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References

Aramideh, SH., Safaralizadeh, M.H., Pourmirza, A.A. & Parvizi, R. 2005. Study on the susceptibility of different larval, prepupa and pupa stages of beet armyworm (spodopetra exigua H.) to steinernema carpocapsae on sugar beet under laboratory conditions. Journal of Agriculture Sciences and Natural Resources, 12(5):159–166.
D–addabbo, T., Carbonara, T., Leonetti, P., Tava, A. & Avato, P. 2011. Control of plant parasitic nematodes with active saponins and biomass from Medicago sativa. Photochemistry Reviews, 10:503–519.
De Geyter, E., Lambert, E., Geelen, D. & Smagghe, G. 2007. Novel advances with plant saponins as natural insecticides to control pest insects. Pest Technology, 1(2):96–105.
Douda, O., 2005. Host range and growth of stem and bulb nematode (Ditylenchus dipsaci) populations isolated from garlic and chicory. Plant Protection Science, 41(3): 104–108.
Fasihi, M., Tanha–Maafi, Z., Kargar–Bideh, A. & Eskandari, A. 2010. Host ranges variability, multiplication and seed–borne ability of some population of stem and bulb nematode (Ditylenchus dipsaci) in IRAN. Iranian Journal Plant Pathology, 46(2): 179–187. (In Persian with English Summary)
Faulkner, L.R., Bower, D.B., Evans, D. & Welgine, J.H. 1974. Mass culturing of Ditylenchus dipsaci to yield large quantities of inoculum. Journal of Nematology, 6(3): 126–129.
Francis, G., Kerem, Z., Makkar, H.P.S. & Becker, K. 2002. The biological action of saponins in animal systems: A review. British Journal of Nutrition, 88: 587–605.
Golawska, S. Leszczynski, B. & Oleszek, W. 2006. Effect of low and high–saponin lines of alfalfa on pea aphid. Journal of Insect Physiology, 52(7): 737–743.
Golawska, S., Lukasik, I., Wojcicka, A. & Sytykiewicz, H. 2012. Relationship between saponin content in alfalfa and aphid development. Acta Biological Cracoviensia Series Botanica, 54(2):39–46.
Hajihassani, A. 2018. Chemical nematicides for control of plant–parasitic nematodes in georgia vegetable crops. UGA Cooperative Extension Bulletin, 1502.
Hajihassani, A., Tenuta, M. & Robert, H.G. 2017. Monoxenic rearing of Ditylenchus weischeri and D. dipsaci and microplot examination of the host suitability of yellow pea to D. weischeri. Plant protection science, 53(4): 254–264.
Hala, S.I., Hamounda, S.E.S., El–Kady, A.M.A. & Abd–Alla, H.I. 2014. Study the nematicidal efficiency of Corchorus olitorius, Cinnamomum Camphora, Portulace and Lantana camara Extracted saponins and their formulations on root–knot nematodes Meloidogyne Spp. Nature and Science, 12(11): 40–45.
Hassan, A., AL–Naser, Z.A. & AL–Asaas, K. 2015. Effect of some plant extracts on larval mortality against the stem nematode (Ditylenchus dipsaci) and compared with synthetic pesticides. International Journal of Chemistry Technique Reseaech, 7(4): 1943–1950.
IPPC. 2016. International standards for phytosanitary measures. ISPM 27 diagnostic protocols, DP 8: Ditylenchus dipsaci and Ditylenchus destructor. International Plant Production Convention, 34 p.
Jordan, S.G. 2018. Modeling the spread of alfalfa stem nematodes: Insights into their dynamics and control. https://digitalcommons.usu.edu/etd/7055.
Kakaee, M. & Mazaheri–Laghab, H. 2016. Biological effects of saponin components extracted from sugar beet (Beta vulgaris L.) leaf on spotted alfalfa aphid (Therioaphis maculate Buckten). Journal of Sugar Beet, 31(2): 189–199. (In Persian with English Summary).
Kakaee, M. & Mazahery–Laghab, H. 2014. Evaluation of alfalfa (Medicago sativa L.) germplasm using multivariate statistical analysis. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 22 (1):125–132. (In Persian with English Summary).
Kamalak, A. & Canobolat, O. 2010. Determination of nutritive value of wild narrow–leaved clover (Trifolium angustifolium) hay harvested at three maturity stages using chemical composition and in vitro gas production. Tropical Grasslands, 44(2): 128–133.
Katarzyna, R., Paweł, P.P., Olga, W., Bogusław, B. & Ryszard, G. 2016. Medicago sativa as a source of secondary metabolites for agriculture and pharmaceutical industry. Phytochemistry Letters, 20: 520–539.
Kiani, G.H. & Abdolahi, M. 2015. Inhibitory effect of aqueous extracts of capparis spinosa flower and ficus carica leaf on meloidogyne incognita, under laboratory condition. Research in plant pathology, 3(1): 37–46 (In Persian with English summary).
Kühnhold, V., Kiewnick, S. & Sikora, R.A. 2006. Development of an in vivo bioassay to identify sugar beet resistance to the stem nematode Ditylenchus dipsaci. Nematology, 8: 641–645.
Lamberti, F., Sasanelli, N., D–Addabbo, T., D–Aloisio, V. & De Cosmus, P. 2001. Chemical treatments and soil solarization for the control of the stem nematode (Ditylenchus dipsaci) on onions. Nematologia Mediterranea, 29: 149–152.
Madani, M., Tenuta, M., Chizhov, V.N. & Subbotin, S.A. 2015. Diagnostics of stem and bulb nematodes, Ditylenchus weischeri and D. dipsaci (Nematoda: Anguinidae), using PCR with species–specific primers. Canadian Journal of Plant Pathology, 37(2): 212–220.
Mazahery–Laghab, H., Yazdi–Samadi, B., Bagheri M. & Bagheri, A.R. 2011. Alfalfa (Medicago sativa L.) shoot saponins: Identification and bio–activity by the assessment of aphid feeding. British Journal of Nutrition, 105: 62–70.
Mervat, I.A.R. & Srour, H.A.M. 2013. Saponins suppress nematode cholesterol biosynthesis and inhibit root knot nematode development in tomato seedlings. Natural Products Chemistry and Research, 2(1): http://dx.doi.org/10.4172/2329–6836.1000123.
Moradi, F., Mazaheri–Laghab, H., Kashi, L. & Moosavi, S.S. 2020. Evaluation of alfalfa ecotypes reactions (Medicago sativa L.) to the stem and bulb nematode (Ditylenchus dipsaci). Iranian Journal of Plant Pathology. 56(2): 153–162. https://doi.org/10.22034/ijpp.2020.46900. (In Persian with English Summary).
Moses, T., Papadopoulou, K.K. & Osbourn, A. 2014. Metabolic and functional diversity of saponins, biosynthetic intermediates and semi–synthetic derivatives. Critical Reviews in Biochemistry and Molecular Biology, 49(6): 439–462.
Mousa, E.M., Mahdy, M.E. & Younis, D.M. 2011. Evaluation of some plant extracts to control root–knot nematode Meloidogyne spp. on tomato plants. Egyptian Journal of Agro Nematology, 10(1): 1–4.
Mugford, S.T. & Osbourn, A. 2012. Saponin synthesis and function. Isoprenoid synthesis in plants and microorganisms. pp. 405– 24.https://doi.org/10.1007/978–1–4614–4063–5.
Nazir, j., Gowen, S.R., El–Hasssan, S.A. & Inam–ul–Haq, M. 2008. Efficacy of neem (Azadirachta indica) formulations on biology of root–knot ematodes (Meloidogyne javanica) on tomato. Crop Protection, 27(1): 36–43.
Nelmes, A.J. 1970. Behavioral responses of Heterodera rostochiensis larvae to aldicarb and its sulfoxide and sulfone. Journal of Nematology, 2: 223–227.
Oka, Y., Koltai, H. & Bar–Eyal, M. 2000. New strategies for the control of plant parasitic nematodes. Pest Management Science, 56: 983–988.
Pederson, M.W., Barnes, D.K., Sorensen, E.L., Griffin, G.D., Nielson, M.W., Hill, J.R.R.R., Frosheiser, F.I., Sonoda, R.M., Hanson, C.H., Hunt, O.J., Peaden, R.N., Elgin, J.R.J.J.H., Anderson, M.J., Goplen, B.P., Elling, L.J. & Howarth, R.E. 1976. Effects of low and high saponin selection in alfalfa on agronomic and pest resistance traits and the inter relationship of these traits. Crop Science, 16(2): 193–199.
Poveda, j., Abril–Urias, P. & Escobar, C. 2020. Biological control of plant parasitic nematodes by filamentous fungi inducers of resistance: Trichoderma, mycorrhizal and endophytic fungi. Froentiers of Microbiology, 11:992. Doi: 10.3389/fmicb.2020.00992.
Tava, A. & Avato, P. 2006. Chemical and biological activity of triterpene saponins from Medicago species. Natural Product Communication, 1(12): 1159–1180.
Ustundag, O. & Mazza, G. 2007. Saponins: Properties, applications and processing. Critical Reviews in Food Science and Nutrition, 47: 231–258.
Wei, L. Ya, N.S., Xi, T.Y., Seo, Y.Y., Suk, J.L., Hyo, J.B., Chang, S.M., Byung, S.H. & Young, H.K. 2013. Isolation of nematicidal triterpenoid saponins from pulsatilla koreana root and their activities against meloidogyne incognita. Molecules, 18: 5306–5316.
Whitehead, A.G. & Hemming, J.R. 1965. A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Annals of Applied Biology, 55: 25–38.
Yavuzaslanoglu, E., Ates Sonmezoglu, O., & Genc, N. 2018. Molecular characterization of Ditylenchus dipsaci on onion in Turkey. European Journal of Plant Pathology, 151(1):195–200.
Yuksel, O., Albayrak, S., Turk, M. & Sevimay, C.S. 2016. Dry matter yields and some quality features of alfalfa (Medicago sativa L.) Cultivars under two different locations of Turkey. Journal of Natural and Applied Sciences, 20(2): 155–160. https://doi/10.19113/sdufbed.25487.
BioControl in Plant Protection
Volume 10, Issue 2
December 2023
Pages 95-107

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