Effect of sulfur and Tree Trichoderma species on egg hatching and mortarity of root–knot nematode (Meloidogyne javanica) under laboratory conditions

Document Type : Research Paper

Authors

1 M.Sc.Department of Plant Protection, Bu–Ali Sina University, Hamedan, Iran.

2 Assistant Professor, Department of Plant Protection, Bu–Ali Sina University, Hamedan, Iran.

3 Professor, Department of Plant Protection, Bu–Ali Sina University, Hamedan, Iran.

10.22092/bcpp.2025.367974.381

Abstract

Root–knot nematode (Meloidogyne sp.) with 105 known species and a wide host range is considered the most damaging plant parasitic nematode. Doing esearch on the control of this nematode without using of chemical pesticides is necessary in order to preserve the environment. The interaction effect of Meloidogyne javanica, sulfur and Trichoderma afroharzianum, T. crassum and two isolates of T. harzianum was investigated in a completely randomized basic design with five replications under laboratory conditions. The effect of fungal extracts at three levels of concentration (basic, 0.1 and 0.01) were investigated in two separate experiments on second stage larval mortality of the nematode at 24, 48 and 72 hours as well as egg hatching after 2, 4, 6 and 8 days. The results of the studies showed that after 72 hours, T. carssum at the basic concentration level had the greatest effect on the mortality of second stage larvae of M. javanica, with a rate of 83.3%. Also, it was showed that T. crassum at the basic level of concentration had the highest effect on inhibiting egg hatching after eight days with a rate of 63.1%.

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