مطالعه فعالیت لیپوکسی‌ژناز و فنیل‌آمونیالیاز در برنج تحت تنش قارچ عامل بیماری سوختگی غلاف، باکتری آنتاگونیست و القاءکننده‌های مقاومت

نوع مقاله: مقاله علمی-پژوهشی

نویسندگان

1 گروه گیاه‎‌پزشکی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

2 گروه گیاه‌پزشکی، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

چکیده

القا مقاومت به بیمارگرها از جمله راه­کارهای مقابله با تنش­های زیستی در گیاهان می باشد. آنزیم­های فنیل­آلانین آمونیالیاز و لیپوکسی­ژناز از جمله آنزیم­های دفاعی قابل القا هستند که در پاسخ به تنش­های زیستی، در گیاه تولید می شوند. این تحقیق با هدف بررسی اثر القاء کننده های زیستی (جدایه­هایی از باکتریsp.  Pseudomonas) و شیمیایی (سیلیکات پتاسیم) بر میزان بیان با استفاده از Real time PCR و تولید آنزیم­های فنیل آلانین آمونیالیاز و لیپوکسی­ژناز پس از ایجاد آلودگی گیاهچه برنج رقم فجر به عامل بیماری سوختگی غلاف انجام شد. نتایج آنالیز بیان ژن­ لیپوکسی­ژناز در زمان‌های صفر، 6، 12، 24، 48 و 72 ساعت بعد از ایجاد آلودگی نشان داد که حداکثر بیان ژن­ در کلیه ترکیبات تیماری در 72 ساعت بعد از آلودگی گیاه مشاهده شده ولی ترکیب باکتری آنتاگونیست و ماده القاکننده سیلیکات پتاسیم در مقایسه با دیگر ترکیبات تیماری در 6 ساعت اولیه آلودگی بیشترین میزان بیان را برای دو آنزیم فنیل آلانین آمونیالیاز و لیپوکسی­ژناز داشتند. آنالیز میزان بیان ژن‌ها با استفاده از روش 2△△CT نیز نشان داد که استفاده از ترکیبات تیماری در بازه زمانی صفر تا 6 ساعت اولیه آلودگی، سبب افزایش معنی دار در میزان پروتئین شدند که این تفاوت در ترکیب تیماری باکتری آنتاگونیست و ماده القاکننده سیلیکات پتاسیم برای آنزیم لیپوکسی­ژناز بیشتر از فنیل‌آلانین آمونیالیاز بود. افزایش بیان این ژن‌ها با افزایش میزان فعالیت آنزیم‌های متناظر ارتباط مستقیم داشت که نشان­دهنده نقش مستقیم ژن­های دفاعی در فعالیت­های سیستم دفاعی گیاه است که با کاربرد سیلیکات پتاسیم و باکتری آنتاگونیست و القاکننده در گیاه می‌توان میزان بیان آن‌ها را افزایش داد.

کلیدواژه‌ها

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