اثرات دوره نوری، زمان انکوباسیون و سرعت چرخش انکوباتور شیکردار در تولید Beauveria bassiana و Metarhizium anisopliae در فرمانتاسیون مایع

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دفتر امور میوه‌های گرمسیری و نیمه گرمسیری، مؤسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

2 پژوهشکده خرما و میوه‌های گرمسیری کشور، مؤسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، اهواز، ایران

چکیده

موفقیت کنترل زیستی آفات با استفاده از قارچ­های بیمارگر حشرات مانند Beauveria bassianaوMetarhizium anisopliae  به روش­های عملی و اقتصادی برای تولید انبوه آن­ها بستگی دارد. در این پژوهش تأثیر دوره نوری (شامل 12-12، 18-6 و 6-18 روشنایی و تاریکی)، زمان انکوباسیون (دوره 48، 72 و 96 ساعت) و سرعت چرخش انکوباتور ( 100، 150 و 200 دور در دقیقه انکوباتور شیکر دار) در تولید بلاستوسپور این دو قارچ در چهار تکرار بررسی شد. نتایج این پژوهش نشان داد که اثر تیمارهای مختلف دوره نوری، دور انکوباتور و دوره انکوباسیون در چرخه تولید بلاستوسپور قارچ­های B. bassianaوM. anisopliae از نظر غلظت اسپور تولیدی، توانایی جوانه­زنی، وزن تر و وزن خشک دارای تفاوت معنی داری در سطح احتمال 1 درصد است. بالاترین غلظت بلاستوسپور، درصد جوانه­زنی، وزن تر و وزن خشک قارچ­های B. bassianaوM. anisopliae در شرایط دوره نوری 18 ساعت روشنایی و 6 ساعت تاریکی، دور انکوباتور 150 دور در دقیقه و دوره انکوباسیون 72 ساعت بود. براساس نتایج این پژوهش دو گونه قارچ بیمارگر می­توانند با استفاده از ترکیب شرایط محیطی و تنظیمات دستگاهی انکوباتور بدست آمده با عملکرد خوبی از تولید و بازده اقتصادی مناسب تولید شوند.

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