تغییرات سلول‌های خونی بید کلم (Lep.: Plutellidae) Plutella xylostella در پاسخ به Bacillus thuringiensis و تنش‌های دمایی

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه صنعتی شاهرود، ایران

چکیده

سیستم گردش خون در انتقال و ذخیرۀ غذا و دفاع در مقابل میکروارگانیسم‌ها فعالیت می‌کند. میزان موفقیت این سیستم وابسته به تعداد و نوع سلول‌های خونی و عملکرد مناسب آن‌ها در برابر عوامل آلودگی در بدن است. در پژوهش حاضر، تاثیرBacillus thuringiensis و تنش‌های دما بر فراوانی هموسیت‌ها و فعالیت فنل اکسیداز لارو سن پنجم بید کلم بررسی شد. در آزمایش اول، برگ‌های کلزا با غلظت زیرکشنده باکتری تیمار شده و لاروهای سن پنج پس از تغذیه از برگ‌ها در فواصل 12 و 24 ساعت خون‌گیری شدند. در آزمایش دوم نیز لاروها به مدت 12 ساعت در دو دمای 4 و 30 درجۀ سلسیوس قرار گرفتند و تنش دما روی هموسیت‌های آن‌ها بررسی شد. نتایج نشان داد که تغذیه از برگ‌های کلزای آلوده به باکتری پس از گذشت 12 و 24 ساعت سبب افزایش معنی‌دار کل سلول‌ها، پلاسموتوسیت‌ها و گرانولوسیت‌ها و فعالیت فنل‌اکسیداز نسبت به شاهد شد. تنش‌های دمایی نیز افزایش فراوانی هموسیت‌ها و فعالیت فنل‌اکسیداز را نسبت به شاهد سبب شدند که این افزایش در لاروهایی که تنش دمای بالا را تحمل کرده بودند، معنی‌دارتر بود. قطعا تحقیقات تکمیلی نیاز است تا بتوان با شناخت ویژگی‌های ایمنی جمعیت‌های حساس و مقاوم بید کلم، امکان استفاده از روش‌های کنترل میکروبی را علیه آن ارزیابی نمود.

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